Expression of α-Amylase Gene of Bacillus licheniformis in Saccharomyces cerevisiae  

作　　者：罗进贤 黎杨 李文清 张添元 何鸣 

机构地区：[1]Department of Biology and Biotechnology Research Center,Zhongshan University,Guangzhou 510275,PRC

出　　处：《Science China Chemistry》1994年第11期1321-1328,共8页中国科学（化学英文版）

基　　金：the State"7.5"Key Project;Science and Technology Commision of Guangdong Province,China

摘　　要：The secretive expression vector has been constructed using the promoter and signal se-quence of yeast MF-α1 factor,and the Bacillus licheniformis α-amylase gene without promoter and signal se-quence has been inserted into the downstream of the signal sequence on the vector.After the readjustment ofthe reading frame,the amylase gene was expressed in Saccharomyces cerevisiae and the product was secretedfrom it.The properties of enzymes secreted from yeast and B.subtilis are compared,and the mechanism ofthe gene expression and product secretion are discussed.The secretive expression vector has been constructed using the promoter and signal se- quence of yeast MF-α1 factor,and the Bacillus licheniformis α-amylase gene without promoter and signal se- quence has been inserted into the downstream of the signal sequence on the vector.After the readjustment of the reading frame,the amylase gene was expressed in Saccharomyces cerevisiae and the product was secreted from it.The properties of enzymes secreted from yeast and B.subtilis are compared,and the mechanism of the gene expression and product secretion are discussed.

关 键 词：Bacillus LICHENIFORMIS Α-AMYLASE gene EXPRESSION SACCHAROMYCES CEREVISIAE 

分 类 号：Q93[生物学—微生物学]