CEREVISIAE

作品数：124被引量：254H指数：9
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Multidimensional Optimization of Saccharomyces cerevisiae for Carotenoid Overproduction被引量：1
《BioDesign Research》2024年第1期20-27,共8页Jian Fan Yang Zhang Wenhao Li Zhizhen Li Danli Zhang Qiwen Mo Mingfeng Cao Jifeng Yuan 
support from the National Natural Science Foundation of China(32270087);the Natural Science Foundation of Fujian Province of China(2020J05011);Guangdong Basic and Applied Basic Research Foundation(2021A1515110340);Xiamen University(0660X2510200);Daan Gene(20223160A0063),and ZhenSheng Biotech.
Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical synthesis.In this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the indu...
关键词：OPTIMIZATION dimension siona multi CAROTENOID CEREVISIAE OVERPRODUCTION SACCHAROMYCES 
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae
《Synthetic and Systems Biotechnology》2024年第4期638-646,共9页Lifang Yu Michael Dare Asemoloye Mario Andrea Marchisio 
Laccase is a multicopper oxidase enzyme that oxidizes a variety of substrates,including polyphenols and polycyclic aromatic hydrocarbons(PAHs).It catalyzes the four-electron reduction of molecular oxygen that results ...
关键词：LACCASE S.cerevisiae Pulse generator Hydrocarbon degradation REPORTER 
Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in S.cerevisiae
《Synthetic and Systems Biotechnology》2024年第2期330-339,共10页Kristin V.Presnell Omar Melhem Sarah M.Coleman Nicholas J.Morse Hal S.Alper 
We acknowledge support from the Air Force Office of Scientific Research under Award No.FA9550-14-1-0089.Sequencing was conducted at the Genomic Sequencing and Analysis Facility(RRID no.SCR_021713);flow cytometry as conducted at the Microscopy and Imaging Facility(RRID no.SCR_021756)at the UT Austin Center for Biomedical Research Support.
Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently(or robustly)with respect to cellular growth phase(exponential and stationary)and fermentation scale(microtiter pla...
关键词：ENDOGENOUS synthetic STATIONARY 
Exploring the potential of Lactobacillus and Saccharomyces for biofunctionalities and the release of bioactive peptides from whey protein fermentate被引量：1
《Food Bioscience》2022年第4期320-338,共19页Chopada Kevalkumar Dineshbhai Bethsheba Basaiawmoit Amar A.Sakure Ruchika Maurya Mahendra Bishnoi Kanthi Kiran Kondepudi G.B.Patil Maunil Mankad Zhenbin Liu Subrota Hati 
Whey protein concentrate-80(WPC-80)fermented with L.fermentum(KGL4)(37℃)and S.cerevisiae(WBS2A)(25℃)was tested for ACE-inhibitory and antioxidant activities over different periods(12,24,36 and 48 h).Proteolytic acti...
关键词：ACE-Inhibitory Antioxidative activity ANTI-INFLAMMATORY SDS FTIR RP-HPLC L.fermentum S.cerevisiae Whey protein fermentate 
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis被引量：2
《Synthetic and Systems Biotechnology》2022年第1期481-489,共9页Yan Wang Hengchang Li Yu Liu Mengyu Zhou Mingzhu Ding Yingjin Yuan 
the National Key Research and Development Program of China(2018YFA0902100);National Natural Science Foundation of China(21676190).
Currently,the establishment of synthetic microbial consortia with rational strategies has gained extensive attention,becoming one of the important frontiers of synthetic biology.Systems biology can offer insights into...
关键词：2-Keto-L-gulonic acid S.cerevisiae K.vulgare Synthetic microbial consortia Transcriptomic analysis Synthetic biology 
Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine被引量：11
《Virologica Sinica》2021年第2期207-219,共13页Chen Chen Deping Hua Jingxuan Shi Zheng Tan Min Zhu Kun Tan Lilin Zhang Jinhai Huang 
supported by the National Key Research and Development Program of China (2018YFD0500500)。
African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high mortality.The primary cellular targets of ASFV is the porcine macrophages.Up to now,no commercial vaccine o...
关键词：African swine fever virus(ASFV) S.cerevisiae Porcine immunoglobulin Fc P30-Fcγ/P54-Fcαfusion proteins 
Expression of fungal biosynthetic gene clusters in S. cerevisiae for natural product discovery
《Synthetic and Systems Biotechnology》2021年第1期20-22,共3页Zihe Liu Zhenquan Lin Jens Nielsen 
This work was supported by National Key Research and Development Program(2018YFA0901800);National Natural Science Foundation of China(22078012);Chinese Postdoctoral Science Foundation(2020M670115);the Novo Nordisk Foundation(NNF10CC1016517),the Knut and Alice Wallenberg Foundation and Beijing Advanced Innovation Center for Soft Matter Science and Engineering.
Fungi are well known for production of antibiotics and other bioactive secondary metabolites,that can be served as pharmaceuticals,therapeutic agents and industrially useful compounds.However,compared with the charact...
关键词：Natural product discovery Cross-species engineering SPLICEOSOME High-throughput BGC cloning Synthetic biology Yeast 
重组酿酒酵母表达幽门螺杆菌VacA蛋白及其免疫原性分析被引量：1
《中国生物工程杂志》2020年第5期15-21,共7页岑黔鸿 高彤 任怡 雷涵 
四川省科技计划重点面上项目(2019YFN0134);中央高校基本科研业务费专项资金(2682018CX73)资助项目。
目的:利用酿酒酵母表面展示技术筛选幽门螺杆菌候选疫苗,并分析其免疫原性。方法:以幽门螺杆菌的空泡型细胞毒素A(vac A)基因作为研究对象,构建重组S.cerevisiae EBY100/pYD1-VacA,通过Western blot、免疫荧光标记和流式细胞仪对S.cerev...
关键词：酿酒酵母表面展示技术 S.cerevisiae EBY100/pYD1-VacA 口服免疫 免疫原性 
Development of Novel Bread by Combining Seaweed Kappaphycus alvarezii from Sri Lanka and Saccharomyces cerevisiae Isolated from Nectarine
《Journal of Agricultural Science and Technology(B)》2019年第5期339-346,共8页Noriko Komatsuzaki Sayoko Arai Shinobu Fujihara Jun Shima Rathnayake Saman Wijesekara MDileepa STde Croos 
Seaweeds are not used as much for edible use although many kinds of seaweed are collected in Sri Lanka.The objective of this study was to develop high quality bread using seaweed,Kappaphycus alvarezii collected from S...
关键词：KAPPAPHYCUS alvarezii SACCHAROMYCES CEREVISIAE organic acids BREAD making 
Strategy for efficient cloning of biosynthetic gene clusters from fungi被引量：2
《Science China(Life Sciences)》2019年第8期1087-1095,共9页Ruixin Li ZiXin Li Ke Ma Gang Wang Wei Li Hong-Wei Liu Wen-Bing Yin Peng Zhang Xing-Zhong Liu 
Filamentous fungi are excellent sources for the production of a group of bioactive small molecules which are often called secondary metabolites(SMs). The advanced genome sequencing technology combined with bioinformat...
关键词：biosynthetic gene CLUSTERS SACCHAROMYCES CEREVISIAE HOMOLOGOUS recombination DNA assembly 
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