Determination of urinary 3-methylhistidine by highperformance liquid chromatography with o-phthaldialdehyde pre-column derivatization  

作　　者：沈敏 袁倚盛 尹路 

机构地区：[1]Department of Instrumental analysis,Jinling Hospital,Nanjing

出　　处：《医学研究生学报》1992年第3期271-275,共5页Journal of Medical Postgraduates

摘　　要：A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation of 3-methylhistidine derivative from other derivativ-es in urine within 25 min.Linearity,recovery and coefficients of vari-ation were studied.The method has been successfully applied to routineanalysis for clinical studies and diagnosis.A high-performance liquid chromatography was describ- ed to measure the concentration of human 3-methylhistidine in urine with o-phthaldialdehyde pre-column derivatization.This assay system resulted in a clear separation of 3-methylhistidine derivative from other derivativ- es in urine within 25 min.Linearity,recovery and coefficients of vari- ation were studied.The method has been successfully applied to routine analysis for clinical studies and diagnosis.

关 键 词：3-methylhistidine HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY 

分 类 号：R[医药卫生]