DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum aestivum L.)  

作　　者：郭光沁 夏光敏 李忠谊 陈惠民 

机构地区：[1]Biology Department, Shandong University, Jinan 250100, PRC

出　　处：《Science China Chemistry》1991年第4期438-445,共8页中国科学（化学英文版）

摘　　要：Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.Protoplasts were isolated from embryogenic calli derived from immature embryos of wheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB medium supplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cells from protoplasts divided to form somatic embryos directly. The somatic embryos grown to 1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor- mones.

关 键 词：WHEAT (Triticum aestivum L.) PROTOPLAST direct somatic EMBRYOGENESIS PLANTLET regeneration 

分 类 号：O6[理学—化学]