原位缺口翻译技术检测大蒜素的抗突变作用  

In Situ Nick Translation Method Reveals the Antimutagenic Effect of Garlic Extract

作　　者：李革[1] 鞠丽梅[1] 欧阳卓志 王红兵[1] 卓燕 蔡有余[1] 

机构地区：[1]中国医学科学院实验动物研究所

出　　处：《中国比较医学杂志》1991年第Z1期69-71,共3页Chinese Journal of Comparative Medicine

摘　　要：采用原位缺口翻译技术,液闪计数法测定,观察了大蒜素拮抗诱变剂丝裂霉素C(MMC)对人外周血细胞的致突变作用,结果表明,大蒜素具有很强的抗突变作用,同时提示此技术是一种快速、敏感、定量检测DNA损伤的方法。The antimutagenic effect of garlic extract on mutagenicity of normal human peripheral blood cells induced by MMC were quantitatively detected by using in situ nick translation method and liquid scintillation counting. The results showed that garlic extract had strong antimutagenicity effect and the nick translation method provided a rapid and sensitive quantitative assay for detecting DNA damage.

关 键 词：原位缺口翻译 大蒜素 抗突变 

分 类 号：R[医药卫生]