Effects of electric field stimulation on endogenous enkephalin content in artery wall  

作　　者：林树新 范瑾之 刘琨 王复周 

机构地区：[1]Department of Physiology,Fourth Military Medical University,Xi'an 710032

出　　处：《Journal of Medical Colleges of PLA(China)》1991年第4期304-308,共5页中国人民解放军军医大学学报（英文版）

摘　　要：After 20min electric field stimulation,L-enkephalin （L-ENK） content in the rabbitear artery declined from the control value of 38.99±17.29 to 12.55±1.96pg/mg of wet tissue（n=12）,and M-enkephalin （M-ENK） content from 134.67±8.11 to 73.68±17.96pg/mg of wet tis-sue （n=6）,both being significantly decreased.This effect was not influenced after blockade ofadrenergic receptor by yohimbine.Contractions of artery strips induced by electric field stimulationwere enhanced distinctly after pretreatment of the artery strips with naloxone,an opioid receptorblocker,and with pronase,but not after pretreatment with trypsin.The results suggested that the re-lease of endogenous enkephalins in the ear artery could be induced by electric field stimulation andx₂-receptor might not participate in the release course,The release amount of ENK from the ar-terv strips increased when the stimulus freduency increased within the range of 2 Hz to 8 Hz.After 20min electric field stimulation,L-enkephalin (L-ENK) content in the rabbit ear artery declined from the control value of 38.99±17.29 to 12.55±1.96pg/mg of wet tissue (n=12),and M-enkephalin (M-ENK) content from 134.67±8.11 to 73.68±17.96pg/mg of wet tis- sue (n=6),both being significantly decreased.This effect was not influenced after blockade of adrenergic receptor by yohimbine.Contractions of artery strips induced by electric field stimulation were enhanced distinctly after pretreatment of the artery strips with naloxone,an opioid receptor blocker,and with pronase,but not after pretreatment with trypsin.The results suggested that the re- lease of endogenous enkephalins in the ear artery could be induced by electric field stimulation and x_2-receptor might not participate in the release course,The release amount of ENK from the ar- terv strips increased when the stimulus freduency increased within the range of 2 Hz to 8 Hz.

关 键 词：ENKEPHALINS RADIOIMMUNOASSAY ARTERY strip electric field STIMULATION 

分 类 号：R[医药卫生]