荞麦芽不同提取部位抗突变作用的实验研究  

Study on the Antimutagenic Effects of Different Extract Parts of Buckwheat Sprout

作　　者：孙国娟[1] 金乾坤[1] 李树锦[2] 李晓东[1] 崔承弼[2] 

机构地区：[1]延边大学基础医学院,吉林延吉133002 [2]延边大学农学院,吉林延吉133002

出　　处：《食品研究与开发》2013年第24期52-55,共4页Food Research and Development

基　　金：国家自然科学基金项目(30960239)

摘　　要：对荞麦芽不同提取部位的抗突变作用进行研究,为继续开发荞麦芽的保健作用提供参考。试验过程中采用改良的Ames试验方法观察荞麦芽不同提取部位的抗突变性。结果:荞麦芽的乙酸乙酯提取物(200μg/plate)对MNNG诱导的沙门氏菌TA98显示了80.6%的抗突变效果,同时乙酸乙酯提取物与其他提取物相比,对4NQO、TrpP-1处理的TA98和TA100显示了更高的抗突变效果,且结果呈现一定的剂量-效应关系。因此表明荞麦芽不同提取部位均具有一定的抗突变性。The antimutagenic effects of different extract parts of buckwheat sprout were studied to provide scientific basis for exploiting health function of buckwheat sprout.respectively ,using Ames test to determine the antimutagenic effects of different extract parts of buckwheat sprout. An ethyl acetate fraction (200μg/plate) from the ethanol extract of buckwheat sprout showed inhibition rate of 80.6%against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 strain. Also the ethyl acetate fraction (200 μg/plate) showed higher antimutagenic activity than other fractions against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO) and 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b) indol (Trp-P-1) in Salmonella typhimurium TA98 and TA100,The results on mechanism suggested that desmutagenicities and bio-antimutagenicities coexisted.Therefore shows that different extract parts of buckwheat sprout has a high resistance to antimutagenicity.

关 键 词：荞麦芽 AMES试验 抗突变 

分 类 号：TS210[轻工技术与工程—粮食、油脂及植物蛋白工程]