食品接触材料中沙门氏菌的多重PCR检测方法  

The Detection Method of Salmonella in Food Packaging Materials

作　　者：赵琢[1] 栗建永[1] 贾晓川[1] 李晶[1] 王华[1] 柳明[1] 

机构地区：[1]天津出入境检验检疫局工业产品安全技术中心,天津300300

出　　处：《食品研究与开发》2013年第24期193-196,231,共5页Food Research and Development

基　　金：国家质检总局科技计划项目(2012IK122)

摘　　要：建立一种PCR方法可以检测食品包装材料中沙门氏菌。参考文献中沙门氏菌的invA基因序列设计引物,通过PCR对沙门氏菌的目的基因进行扩增,并与其它常见致病菌同时进行多重PCR检测。能特异地扩增出284 bp的目的条带,检出限可达到0.855 1 pg。该方法具有良好的检测特异性,可实现沙门氏菌的快速检测。The purpose of this paper is to establish a PCR method in which Salmonella can be detected in food packaging materials. The primers were designed according to invA gene as references , and we amplified the target gene by single and multiple PCR. Since the detection limit can reach 0.855 1 pg , the detected method is very specific, and can achieve the rapid detection of Salmonella in food packaging materials.

关 键 词：PCR 食品接触材料 沙门氏菌 

分 类 号：O657[理学—分析化学] TS206[理学—化学]