Intein-mediated purification system: mechanism and applications  

作　　者：Sarra setrerrahmane Shuhua Tan 

机构地区：[1]China Pharmaceutical University

出　　处：《科学时代》2013年第7期-,共6页SCIENCE TIMES

摘　　要：The incorporation of self-cleaving protein elements into a variety of fusion-based purification systems; has been an important development in the area of recombinant protein purification. The self-cleaving capability of these tags has recently been combined with additional purification tags to generate novel and convenient protein purification methods. This review elucidates the properties of intein, the mechanism of the intein-based protein splicing and the progress of intein-based protein purification procedures, and recent advances in the applications of intein.

关 键 词：self-cleaving FUSION PROTEIN PURIFICATION INTEIN 

分 类 号：TQ0[化学工程] R73[医药卫生—肿瘤]