Insight into the mechanisms and functions of spliceosomal snRNA pseudouridylation  

作　　者：Hironori Adachi Yi-Tao Yu 

机构地区：[1]Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester Medical Center, Rochester, NY 14642, United States

出　　处：《World Journal of Biological Chemistry》2014年第4期398-408,共11页世界生物化学杂志（英文版）（电子版）

基　　金：Supported by Grants from the National Institute of Health to YiTao Yu,No.GM104077 and AG39559;by the University of Rochester CTSA award(to Yi-Tao Yu)from the National Center for Advancing Translational Sciences of the National Institute of Health,No.UL1TR000042

摘　　要：Pseudouridines(Ψs) are the most abundant and highly conserved modified nucleotides found in various stable RNAs of all organisms. Most Ψs are clustered in regions that are functionally important for pre-m RNA splicing. Ψ has an extra hydrogen bond donor that endows RNA molecules with distinct properties that contribute significantly to RNA-mediated cellular processes. Experimental data indicate that spliceosomal sn RNA pseudouridylation can be catalyzed by both RNA-dependent and RNA-independent mechanisms. Recent work has also demonstrated that pseudouridylation can be induced at novel positions under stress conditions, suggesting a regulatory role for Ψ.Pseudouridines(Ψs) are the most abundant and highly conserved modified nucleotides found in various stable RNAs of all organisms. Most Ψs are clustered in regions that are functionally important for pre-m RNA splicing. Ψ has an extra hydrogen bond donor that endows RNA molecules with distinct properties that contribute significantly to RNA-mediated cellular processes. Experimental data indicate that spliceosomal sn RNA pseudouridylation can be catalyzed by both RNA-dependent and RNA-independent mechanisms. Recent work has also demonstrated that pseudouridylation can be induced at novel positions under stress conditions, suggesting a regulatory role for Ψ.

关 键 词：Pre-m RNA SPLICING U2 sn RNA Box H/ACA RIBONUCLEOPROTEIN PSEUDOURIDINE Induced RNA modification 

分 类 号：Q522[生物学—生物化学]