云南乌金猪与约大乌猪低氧适应性的研究  

The Study of Hypoxia Adaptive Differences of Yunnan Wujin and Yuedawu Pigs

作　　者：李美荃[1] 张春勇[1] 安清聪[1] 潘洪彬[1] 陈克嶙[1] 郭荣富[1] 

机构地区：[1]云南农业大学动物科学技术学院云南省动物营养与饲料重点实验室,昆明650201

出　　处：《畜牧兽医学报》2015年第6期965-973,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：云南省重大科技专项项目(2012ZA018);云南现代农业生猪产业技术体系(A3007381)

摘　　要：旨在探明云南高原乌金猪与约大乌猪低氧适应差异。采集60日龄乌金猪与约大乌猪各30头(公母各半)血样,检测乌金猪和约大乌猪血液血红蛋白含量(HGB)、红细胞压积(HCT)、红细胞数目(RBC)、平均血红蛋白含量(MCH)、平均血红蛋白浓度(MCHC)和氧饱和度生理表征参数。屠宰60日龄乌金猪与约大乌猪各12头(公母各半),采用Real-time PCR方法检测心、肝、肺、肾、空肠、回肠、十二指肠、皮肤、肌肉、胰腺、睾丸或卵巢12种组织中HIF-1α、VEGF、EPO基因的表达。结果显示,乌金猪HGB和RBC极显著(P<0.01)高于约大乌猪,而HCT和MCH低于约大乌猪,乌金猪氧饱和度高于约大乌猪,但差异不显著(P>0.05)。在乌金猪与约大乌猪的各个组织中均检测到HIF-1α、VEGF、EPO基因mRNA的表达;在低氧适应基因中,HIF-1α基因在肝、肺、胰腺、肾和皮肤组织表达量最高,VEGF基因在肝、肺和胰腺表达量最高,EPO基因在肾和肝表达量最高;HIF-1α、VEGF、EPO基因在两猪种中的表达量趋势一致,约大乌猪组织中的总体表达量高于乌金猪的表达量;母猪低氧适应基因的总体表达量显著高于公猪。乌金猪低氧适应生理表征参数明显高于约大乌猪,在多数组织约大乌猪低氧适应基因的表达高于乌金猪。但对其高原低氧适应性机制有待进一步研究。This study aimed to ascertain the differences of hypoxia adaptation between Yunnan plateau Wujin pig and Yuedawu pig.Blood samples of 30 Wujin pigs and 30 Yuedawu pigs were collected(Half male and female)on 60 days.The blood HGB,HCT,RBC,MCH,MCHC and oxygen saturation were determined.The gene expressions of HIF-1α,VEGF and EPO were determined by real-time PCR in heart,liver,lung,kidney,jejunum,ileum,duodenum,skin,muscle,pancreas,testes and ovaries of Yuedawu and Wujin pigs(half male and female).The results showed that HGB and RBC of Wujin pigs was significant higher than that of Yuedawu pigs(P<0.01),and hematocrit(HCT)and MCH was lower than Yuedawu pigs.Oxygen saturation of Wujin pigs was higher than that of Yuedawu pigs,without significant differences(P>0.05).HIF-1α,VEGF,EPO mRNA were detected in various tissues of Yuedawu and Wujin pigs.The gene expressions of HIF-1α,VEGFand EPOin most tissues of Yuedawu pigs were higher than that in Wujin pigs,and main expression tissues for HIF-1αand VEGF were liver,lungs and pancreas in 2pig breeds,whereas EPO was mainly expressed in kidney and liver,the trends of HIF-1α,VEGF and EPOgenes expression in 2pig breeds is consistent.In 2pig breeds,the hypoxia adaptation gene expressions of sows were significantly higher than that of boar.The HGB,RBC and oxygen saturation of Wujin pigs was significantly higher than that of Yuedawu pigs.Physiological characterization parameters of hypoxia adaptation of Wujin pig were significantly higher than Yuedawu pig.Hypoxia adaptation gene expression in most tissues of Yuedawu pig were higher than that in Wujin pig.But the mechanisms of hypoxia adaptation need to be researched in the further.

关 键 词：乌金猪 约大乌猪 HIF-1Α VEGF EPO 基因表达 

分 类 号：S828[农业科学—畜牧学]