1-MCP对CO_2处理‘磨盘柿’果实活性氧含量及清除酶活性的影响  

Influence of 1-MCP on reactive oxygen species content and scavenger enzyme activity in persimmon fruit treated with CO_2

作　　者：李莹[1] 程青[1] 李冉[1] 何水仙[1] 李宝[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《果树学报》2015年第3期494-501,共8页Journal of Fruit Science

基　　金：公益性行业(农业)科研专项经费(201203047)

摘　　要：【目的】探究活性氧在柿果实采后软化的作用。【方法】以房山‘磨盘柿’为材料,研究室温下柿果实采后经CO2、CO2+1-MCP处理对其软化过程中活性氧含量及其清除酶活性变化的影响。【结果】采后柿果实CO2处理后在贮藏期间硬度迅速下降,呼吸速率和乙烯释放率加快;同时·OH、O2·和H2O2含量迅速积累,其清除酶SOD活性先下降后迅速上升,POD、CAT和APX活性迅速上升。CO2+1-MCP处理能有效明显降低呼吸速率和乙烯释放率且推迟了乙烯峰的出现;·OH积累速度差异不大,但极大的延缓了O2·和H2O2的积累速度;SOD、POD、CAT和APX活性较CO2也明显降低。【结论】柿果实经CO2、CO2+1-MCP处理后在软化过程中加速了活性氧的积累,其可能是影响果实快速软化的重要因素。同时1-MCP加入有效降低了活性氧的积累速度,对保持果实硬度起到一定的作用。【Objective】To explore the role of active oxygen in the softening process of persimmon fruit af-ter postharvest.【Methods】1-MCP was applied to investigate its effects on reactive oxygen species(ROS)content and scavenger enzyme activity in persimmon(Diospyros kaki Thunb.‘Mopanshi')fruit treatedwith CO2. The treatments included Control,CO2 and CO2+ 1-MCP.【Results】The results indicated thatthe treatment of CO2 led to rapidly loss of fruit firmness,the increase of respiration rate and ethylene re-lease rate,the quick accumulation of ·OH,O2·and H2O2 and the increase of SOD,POD,CAT and APXactivity. Compared with treatment of CO2,the combination of CO2+1-MCP could effectively keep the fruitfirmness,decrease respiration rate and ethylene release rate,delay the appearing timing of respiratorypeak and ethylene peak,reduce ROS accumulation rate and inhibit scavenger enzymes activity.【Conclu-sion】The results suggested that treatment of CO2 and CO2+1-MCP could result in the advance of the fruitsoftening and enhance ROS accumulation. 1-MCP could effectively reduce the ROS accumulation rate,delay softening of fruit and prolong shelf life.

关 键 词：'磨盘柿’ 1-MCP CO2 果实软化 活性氧 

分 类 号：S665.2[农业科学—果树学]