毛细管区带电泳法测定单唾液酸神经节苷脂GM_1的含量  

Separation and determination of content of monosialogangliosides GM_1 by capillary zone electrophoresis

作　　者：郑永彪[1] 范慧红[1] 丁春玲 徐康森[1] 

机构地区：[1]中国药品生物制品检定所 [2]承德露露股份有限公司,河北承德067000

出　　处：《中国生化药物杂志》2004年第3期139-141,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：北京市自然基金资助项目 ( 70 32 0 4 1 )

摘　　要：目的建立毛细管区带电泳法分离测定单唾液酸神经节苷脂GM1 的方法。方法采用熔融的硅胶毛细管柱 (90cm× 75 μm ,有效长度为 83cm) ,以含 1 5mmol/L浕 环糊精的 2 5mmol/L硼砂缓冲液为电极缓冲液 (pH 9.4 ) ,检测波长 :2 0 0nm。结果GM1 线性范围为 0 .0 4 2～ 4 .2 3mg/ml(r =0 .9981 ) ,回收率 (n =3)分别为 98.85 % (RSD =1 .7% )、99.0 6 % (RSD =1 .1 % )、99.0 1 % (RSD =1 .2 % )。结论此法简便、快速、准确 。PurposeTo establish a method using capillary zone electrophoresis for separating and determining the content of GM 1.MethodsOn Fused Silica capillaries (90 cm×75 μm efficiency length 83 cm),using 25 mmol/L borax buffer containing 15 mmol/L ɑ cyclodextrin at pH 9.4, the UV detection wavelength was 200 nm.ResultsThe average recoveries of GM 1 ( n =3) were 98.85%( RSD =1.7%),99.06%( RSD = 1.1% ),99.01%( RSD =1.2%) respectively. The calibration curves were linear in the range of 0.042～ 4.23 mg/ml( r =0.998 1).ConclusionThis method is simple, quick, accurate and very suitable for the quality control of the drug preparation manufacturing and clinical application.

关 键 词：单唾液酸神经节苷脂 毛细管区带电泳 Α-环糊精 

分 类 号：R927.2[医药卫生—药学] R971