喉癌STK15基因异常与中心体扩增的研究  

Study on STK15 gene abnormality and centrosomal amplification in laryngeal carcinoma

作　　者：李英慧[1] 李福才[1] 王曦[2] 赵旭[1] 叶燕[1] 孙兴和 孙开来[1] 

机构地区：[1]中国医科大学医学遗传学教研室,沈阳110001 [2]武警辽宁省总队医院外一科 [3]空军463医院耳鼻喉科

出　　处：《中华医学遗传学杂志》2004年第3期240-244,共5页Chinese Journal of Medical Genetics

基　　金：辽宁省自然科学基金 (2 0 0 1 1 0 1 0 39);国家自然科学基金(30 1 71 0 0 8)~~

摘　　要：目的　研究喉癌中 STK15基因的异常及中心体扩增情况。方法　应用逆转录 -聚合酶链反应方法 ,检测 6 2例喉鳞状细胞癌和人喉鳞状细胞癌细胞系 Hep- 2中 STK15基因 m RNA表达水平 ;应用聚合酶链反应 -单链构象多态性检测上述组织及细胞中 STK15基因第 6、7外显子突变 ;以 Hep- 2细胞系为代表应用免疫荧光方法检测中心体扩增情况。结果　在 39例 ( 6 3% )喉癌及 Hep- 2细胞系中检测到了STK15基因的高表达 ;在上述组织和细胞中均未检测到 STK15基因第 6、7外显子的突变 ;Hep- 2细胞系有明显的中心体扩增 :单个细胞中心体数目变化范围为 1～ 7,有中心体扩增的细胞数为 11%～ 2 3%。结论　在 Hep- 2细胞系中发现了 STK15基因高表达与中心体扩增 ,提示 STK15基因高表达引起中心体扩增 ,可能是导致该细胞系染色体不稳定的重要因素 ;在喉癌组织中观察到了STK15基因高表达 。Objective To investigate STK15 gene abnormality and centrosomal amplification in laryngeal carcinoma. Methods STK15 gene mRNA expressional level was tested in 62 cases of laryngeal squamous cell carcinoma and laryngeal squamous cell carcinoma cell line Hep-2 by reverse transcription-polymorase chain reaction(RT-PCR); the mutation of STK15 gene exon 6 and exon 7 in the same tissues and cells was detected by PCR-single strand conformation polymorphism. Immunofluorescent antibodies were used to test centrosomal amplification in Hep-2 cell line as an example. Results STK15 gene overexpressed in 39 cases of laryngeal carcinoma (63%) and Hep-2 cell line. No mutation was found in exon 6 and exon 7 of STK15 gene in the above tissues and cells. Centrosomal amplification was apparent in Hep-2 cell line. The number of centrosome in a single cell changed from 1 to 7, and Hep-2 cells with amplified centrosomes (more than 2 in one cell) were 11%-23%. Conclusion STK15 gene overexpression and centrosomal amplification were first found in human laryngeal squamous cell carcinoma, which indicated that STK15 gene overexpression leading to centrosomal amplification might occur in the early stage of human laryngeal carcinogenesis and be one of the key mechanisms for the occurrence of laryngeal carcinoma.

关 键 词：喉癌 STK15基因 基因异常 中心体扩增 

分 类 号：R739.65[医药卫生—肿瘤]