X线修复交叉补体因子1多态性与肝细胞癌风险的关系  

POLYMORPHISM OF DNA REPAIR GENE XRCC1 AND RISK OF HEPATOCELLULAR CARCINOMA

作　　者：龙喜带[1] 马韵[2] 韦义萍[3] 邓卓霖[2] 

机构地区：[1]广西医科大学 [2]广西医科大学病理学教研室,南宁530021 [3]广西医科大学护理学院病理学教研室

出　　处：《广西医科大学学报》2004年第3期313-315,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目 ( No.3 9860 0 3 2 )

摘　　要：目的 :研究广西地区人群 DNA修复酶 X线修复交叉补体因子 1 (XRCC1 ) codon 399Gln多态性与肝细胞癌 (HCC)易感性的关系。方法 :采用外周血白细胞 DNA进行 PCR- RFL P检测分析 1 4 0例 HCC患者和 5 36例对照人群 XRCC1基因 399位点多态性。结果 :1 XRCC1 codon 399Arg/Arg为 HCC保护基因型 ,XRCC1 codon 399(Gln/Gln+Arg/Gln)为 HCC风险基因型 (校正 OR=2 .1 8,95 % CI=1 .2 7～ 3.74 ) ;2在 AFB1 暴露有中低度与高度两个层次 ,XRCC1 399Gln多态性在 AFB1 中低度暴露群体中增加 HCC危险性 (校正 OR=2 .0 6 ,95 % CI=1 .0 1～ 4 .2 0 )。结论 :XRCC1 399Gln多态性与 HCC风险相关 ,且在 AFB1 中低度暴露时更易感 HCC。Objective:To determine whether the XRCC1 codon 399 Gln allele is a risk factor for HCC in Guangxi population.Methods:The peripheral blood white blood cells DNA samples were obtained from all the subjects including 140 HCC cases and 536 controls. The XRCC1 gene 399 codon polymorphism were detected by PCR RFLP technique.Result:a.The XRCC1 399 Arg/Arg is associated with decreasing HCC risk,while the XRCC1 399 (Gln/Gln & Arg/Gln) is associated with increasing HCC risk (adjusted OR=2 18, 95%CI=1 27~3 74); b. In the cohort of low/median level of AFB 1 exposure, the codon 399 Gln allele is associated with a conspicuous increasing risk for HCC (adjustd OR=2 06, 95%CI=1 01~4 20).Conclusion:The XRCC1 399 polymorphism is associated with the risk of HCC. There is evidence for interaction between XRCC1 399 Gln allele and AFB 1exposure, especially with low/mediun degrees of AFB 1 exposure.

关 键 词：X线修复交叉补体因子1 多态性 黄曲霉毒素B1 DNA损伤 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]