Kluyveromyces fragilis LFS-8611 β-D-半乳糖苷酶的摇瓶培养条件  

Culture Conditions for β-D-galactosidase Prodution byKluyveromyces fragilis LFS-8611

作　　者：刘建福[1] 王璋[1] 许时婴[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第4期82-85,93,共5页Journal of Wuxi University of Light Industry

摘　　要：脆壁克鲁维酵母(Kluyveromycesfragilis)LFS 8611合成的β D 半乳糖苷酶具有较高的催化半乳糖基转移反应活力.脆壁克鲁维酵母(K.fragilis)LFS 8611细胞生长和β D 半乳糖苷酶的合成同步.该菌株生长和产酶的最适碳源为半乳糖,乳糖次之;最适氮源为蛋白胨F403;最适培养条件为:发酵培养基的初始pH值为7.0,摇床的转速为200r/min.培养基中碳源和氮源质量浓度对菌体生物量和β D 半乳糖苷酶活力有重要影响,以12mg/mL乳糖为碳源,16mg/mL蛋白胨(F403)为氮源,在最适培养条件下培养32h后,菌体生物量和β D 半乳糖苷酶活力分别为7.56g/L和18.83U/mL.Kluyveromyces fragilis LFS-8611 produced an intracellular β-D-galactosidase with high tansglactosylation activity. Kluyveromyces fragilis LFS-8611 growth was associated with β-D-galactosidase synthesis.Galactose and peptone F403 were the best carbon and nitrogen soureces for Kluyveromyces fragilis growth and enzyme synthesis,followed by lactose(as C source). The optimal culture conditions for the growth and enzyme production with the strain was initial pH 7.0 and rotation speed 200 r/min. Biomass and β-D-galactosidase activity significantly decreased when culturing the strain in lower C or N sources. The biomass and enzymatic activity reached 5.89 g/L and 18.83 U/mL respectively,after 32 h cultivation under optimal culture conditions.

关 键 词：脆壁克鲁维酵母(Kluyveromyces fragilis) β-D-半乳糖苷酶合成 培养条件 

分 类 号：TQ925[轻工技术与工程—发酵工程]