紫金牛地上和地下部位挥发性成分比较研究  

Analysis of Volatile Oils from Ardisia japonica (Homsted) BL. between Aerial and Underground Parts

作　　者：倪士峰[1] 黄静[1] 潘远江[1] 傅承新[2] 吴平[2] 

机构地区：[1]浙江大学理学院化学系有机研究所 [2]浙江大学生命科学学院植物科学研究所,杭州310029

出　　处：《药物分析杂志》2004年第3期257-261,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:研究紫金牛生药地上部位、地下部位挥发油成分。方法:采用水蒸气蒸馏法分别提取紫金牛地上部分和根部的挥发油。用GC毛细管柱进行分离分析,并用气相色谱及气相-质谱法对化学成分进行鉴定。色谱条件:Varian 3300气相色谱仪,HP-innowax型石英毛细管柱(30 m×0.25cm×0.25μm)。载气为高纯氦气,FID检测器。质谱条件:Saturn 2000质谱仪(Varian,USA),EI电离源,电离电压70 eV,离子源温度200℃,接口温度240℃,扫描范围:40-450 amu,进样量1.0μL,分流比10:1。结果:本研究从紫金牛地上部分鉴定出了61个组分,占挥发油总量的87.65%,含量在4%以上的组分有:芳樟醇(4.20%)、石竹烯(4.26%)、苯乙醇(4.38%)、水杨酸甲酯(4.47%)、兰桉醇(5.07%)、α-石竹烯(5.14%)、己酸(9.10%)、3,7,11-三甲基-1,6,10-十二碳三烯-3-醇(14.07%)。从根部共鉴定出45个组分,占挥发油总量的88.17%,含量在4%以上的组分有:丁子香基乙醇(4.22%)、兰桉醇(6.02%)、α-杜松醇(8.90%)、龙脑(9.16%)、α-石竹烯(12.89%)、石竹烯(30.13%)。结论:从其不同部位成分比较来看,紫金牛地上部分和地下部分要区别对待,分别人药。Objective:To provide the foundation for reasonable utilization,essential oils from Ardisia japonica between aerial parts and undergrounds parts were analyzed. Methods: Essential oils were obtained by steam distillation. The chemical components were separated and identified by gas chromatography (GC) and gas chromatogra phy - mass spectrometry (GC-MS). The relative content of each component was determined by area normalization. Results:Sixty - one peaks were identified from aerial parts of this plant, which resenting 87. 65% of the total oil. Main constituents of the essential oil were found to be methyl salicylate(4.47%) ,globulol(5.07%),3,7,11-trimethyl-1,6,10 -dodecatrien -3 -ol(14. 07% ) ,α-caryophyllene(5.14% ) ,capronic acid(9. 10% ). Forty -five peaks were i'dentified from root oil,which resenting 88. 17% of the total oil. Main constituents of the essential oil were found to be globulol(6. 02% ) ,α- cadinol(8. 90% ) ,borneol(9.16% ) ,α- caryophyllene (12. 89% ) , caryophyllene(30.13%). Conclusion: Different parts of this medical herb possess different essential substances in quantitative and qualitative in different stage,and should be regarded as different drug in clinical application.

关 键 词：紫金牛 挥发性成分 气相色谱-质谱 挥发油 植物药 

分 类 号：R282.71[医药卫生—中药学]