N^+注入引起向日葵蛋白质组变异研究初报  

THE ANALYSIS OF PROTEOME CHANGES IN SUNFLOWER SEEDS INDUCED BY N+ IMPLANTATION

作　　者：董贵俊[1] 张卫东[1] 陈双燕[1] 齐冬梅[1] 常凤启[1] 朱至清[1] 刘公社[1] 余增亮[2] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093 [2]中国科学院等离子体物理所离子束生物工程学重点实验室,合肥230031

出　　处：《生物物理学报》2004年第4期269-274,共6页Acta Biophysica Sinica

基　　金：中国科学院知识创新工程十五重大项目(交叉项目)

摘　　要：低能离子注入可以引起生物DNA分子结构的变异,但用蛋白质组学方法对低能离子注入后所引起的蛋白质诱变效应的研究尚缺乏文献报道。以向日葵种子为实验材料,对N+注入后种子的蛋白质组进行了初步分析, 共获得369个蛋白质点,其中包括对照种子中的8个特异点和处理种子中的4个特异点。这些特异点的分子量在15～34 ku之间。通过基质辅助激光解吸电离飞行时间质谱鉴定发现对照种子蛋白质中的No.29特异蛋白与MADS盒转录因子HAM59有23.48%的匹配率。处理种子中的No.279特异蛋白与亮氨酸拉链蛋白的同源蛋白HAHB-4有23.20%的匹配率。The changes of proteins induced by N+ implantation were investigated using a sunflower seed model. To better understand changes of total protein irradiated with N+, a proteomic analysis of N+ implantation in seeds were developed. 369 total proteins were obtained in two-dimensional gels, including 8 protein spots in untreated seeds and four spots in treated seeds respectively. Furthermore,6 proteins were selected and identified using MALDI-TOF MS and only two of them had been reported. The coverage between the protein in untreated seeds (No.29) and MADS-box transcriptional factor HAM59 was 23.48%, and the other was 23.20% between the protein in treated seeds (No.279) and homeobox-leucine zipper protein HAHB-4.

关 键 词：离子注入 种子 蛋白质组 双向电泳 质谱 

分 类 号：Q3[生物学—遗传学]