细菌脂多糖诱导人胃上皮癌细胞株BGC-823环氧化酶-2的合成  

Lipopolysaccharide induces the synthesis of cyclooxygenase-2 in human gastric carcinoma epithelial cell line BGC-823

作　　者：李莲[1] 冯桂香[1] 任永生[1] 王伟[1] 熊顺华[1] 

机构地区：[1]郧阳医学院生理学教研室,湖北十堰442000

出　　处：《郧阳医学院学报》2004年第2期85-87,共3页Journal of Yunyang Medical College

基　　金：湖北省教育厅科研基金资助项目(鄂教科 [2 0 0 3]5号 )

摘　　要：目的 :研究细菌脂多糖 (lipopolysaccharide,LPS)对人胃上皮细胞环氧化酶 1和环氧化酶 2合成的影响。方法 :实验选用LPS(E .Coli 0 111∶B4 )和人胃上皮癌细胞株BGC - 82 3。环氧化酶的合成用免疫蛋白印迹法(WesternBlotting)测定。 结果 :LPS对BGC 82 3细胞环氧化酶 2的合成有明显的诱导作用。BGC - 82 3细胞环氧化酶 - 2的含量在LPS作用 1h后明显增加 ,并持续至少 6h。结论 :E .Coli的LPS可诱导BGC - 82 3细胞COX- 2的合成。Objective To investigate the effect of lipopolysaccharide (LPS) on the synthenase of cyclooxygenase-1 and cyclooxygenase-2 in human gastric epithelial cell line, BGC-823. Methods LPS (E. Coli 0111:B4) and BGC-823 were used in our study. The synthesis of cyclooxygenase was examined by Western blot. Results The results of Western blot showed that the content of cyclooxygenase-2 increased obviously after the cells incubated with lipopoly saccharide a hour and it could last for six hours at least, the quantity of COX-1 did not change during this period. Conclusions E. Coli LPS would be effective stimuli of the synthesis of COX-2 in gastric carcinoma epithelial cell line.

关 键 词：细菌 脂多糖 胃癌 上皮癌细胞 环氧化酶 LPS COX 细胞培养 免疫蛋白印迹法 

分 类 号：R735.2[医药卫生—肿瘤]