酯化前后酵母菌的红外光谱比较  

Comparison of Infrared Spectra of Native and Esterified Beer Yeast

作　　者：韩润平[1] 鲍改玲[1] 朱路[1] 

机构地区：[1]郑州大学化学系,河南郑州450052

出　　处：《光谱学与光谱分析》2004年第7期820-822,共3页Spectroscopy and Spectral Analysis

基　　金：河南省科技攻关项目 (981 1 60 4 1 6)资助

摘　　要：对啤酒酵母酯化前后的红外光谱进行了分析。啤酒酵母的红外光谱图主要由蛋白质的吸收带、碳水化合物的吸收带组成。16 5 2cm- 1 处的吸收峰为酰胺Ⅰ带 ,是CO的伸缩振动 ,15 4 2cm- 1 的吸收峰是酰胺Ⅱ带 ,是N—H的弯曲振动和C—N的伸缩振动 ,12 4 0cm- 1 是酰胺Ⅲ带 ,是C—N的伸缩振动和N—H的弯曲振动引起的。14 5 4cm- 1 处的吸收峰为CH3和CH2 的弯曲振动峰 ;116 0cm- 1 处出现的峰可能为细胞壁的主要成分———碳水化合物中C—O的伸缩振动峰 ;10 80cm- 1 处的吸收峰是由啤酒酵母中的RNA ,DNA或细胞壁中存在的碳水化合物或醇中的C—O伸缩振动引起的。用甲醇酯化后在 174 4和 14 5 4cm- 1 处的吸收峰强度增加 ,说明酵母菌细胞表面的羧基发生了酯化反应。酯化后细胞的主要成分和结构保持完整。The native beer yeast and esterified beer yeast were examined by infrared spectroscopy. The IR spectrum of beer yeast is mainly composed of the adsorption of carbohydrates, protein, etc. The dominating bands near 1652, 1 532 and 1240 cm were assigned to amide I, amide H and amide III, and the characteristic IR absorption of protein could be one of the significant components of cell walls. The peak near 1454 cm(-1) is attributable to the bending stretching of CH2- and CH3-. A substantial portion of the absorbance at 1 160 cm(-1) is attributable to the stretching vibration of C-O on the structure of carbohydrates, the main components of the cell walls. The band present at 1080 cm(-1) was caused by the C-O stretching of carbohydrates and alcohols found in the RNA, the DNA and/or the cell envelop of the yeast. The peaks at 1744 cm(-1) (attributed to the carboxylate stretching) and 1454 cm(-1) confirmed the esterification process of carboxylate groups presented in the cell wall. After esterification with methanol-chloride hydride, the major components and the structures of the biomaterial remained intact.

关 键 词：红外光谱 酯化 啤酒酵母 羧基 细胞成分 细胞结构 重金属 吸附作用 

分 类 号：X172[环境科学与工程—环境科学]