蛋白芯片法检测多肿瘤标志物的临床应用  

Clinical application of a micro-array for multi-tumor marker detection

作　　者：兰小鹏[1] 赵猛[1] 马立强[1] 薛昭卿[1] 

机构地区：[1]南京军区福州总医院解放军医学检验中心,福建福州350025

出　　处：《医学研究生学报》2004年第8期716-719,共4页Journal of Medical Postgraduates

摘　　要：目的 :探讨多肿瘤标志物联合检测在肺癌、肝癌、胰 /结直肠癌及胃癌等四种肿瘤中的临床应用价值。　方法 :采用多肿瘤标志物蛋白芯片法 (C 12系统 )联合检测 30例肺癌、19例肝癌、2 4例胰 /结直肠癌和 2 2例胃癌及173例良性疾病患者 ,并对结果进行敏感度 /特异性 (ROC)分析。　结果 :C 12系统中单个标志物在四种肿瘤中的检出率与文献报道无显著差异 ;肿瘤组的阳性率明显高于非肿瘤组 (分别为 85 .3%和 18.5 % ;P <0 .0 0 1) ,而且肿瘤组阳性平均值明显高于非肿瘤组 (P <0 .0 1) ;系统整体ROC曲线下面积明显大于任何单项标志物。　结论 :C 12系统在肿瘤标志物检测上有较高的灵敏度和较好的特异性 ,其临床应用价值较高。Objective: To investigate the clinical value of a micro-array for multi-tumor marker detection (abbreviate C-12 system in the following) in four kinds of tumors (lung, liver, pancreas/colon and stomach cancers). Methods:30 lung cancer?19 liver cancer?24 pancreas/colon?22 stomach cancer and 173 non-tumor patients' serum were detected by C-12 system, and the results were analyzed by ROC curve.Results:There is no difference in the positive rate of single TM between C-12 system and the previously reports; The positive rate and the mean positive value of tumor patients were both obviously higher than that of non-tumor patients (P<0.001 and P<0.01 respectively); The area of below ROC curve of the whole system is outstanding bigger than that of any single TM in these four kinds of tumor. Conclusion:The micro-array is stable, sensitive, specific, and can be used as a routine assay for detection the TM in the clinic.

关 键 词：肿瘤标志物 蛋白芯片 ROC 

分 类 号：R730.43[医药卫生—肿瘤]