一个新的基因转录本的克隆及序列分析  

Cloning and sequence analysis of a novel gene transcript

作　　者：顾爱华[1] 周作民[1] 沙家豪[1] 

机构地区：[1]南京医科大学生殖医学实验室,江苏南京210029

出　　处：《东南大学学报（医学版）》2004年第4期214-216,共3页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目 (30 1 70 4 85);国家重点基础研究发展规划 973项目 (G1 9990 5590 1 )

摘　　要：目的 :筛选与精子发生和 (或 )睾丸发育相关的基因。方法 :将不同发育阶段 (胚胎和成人 )的睾丸组织cDNA探针与人睾丸cDNA芯片进行杂交 ,通过杂交信号的比较 ,筛选出差异表达的基因。结果与结论 :经测序发现该基因全长 13 16bp ,包含1个 10 5 6bp的开放阅读框 ,编码 3 5 1个氨基酸。生物信息学序列分析表明 ,它是p44S10基因家族的一条新的转录本基因 ,该基因的氨基酸序列编码一 2 6S蛋白酶体调控亚基 ,在进化中高度保守 。Objective To identify genes involved in spermatogenesis and testicular development.Methods Hybridization of human adult and fetal testes cDNA samples was performed with a human cDNA microarray.Results and Conclusions By analysing the hybridization signals,a gene with a differential expression in adult and fetal testis was identified.The full length cDNA of 1?316?bp contained an open reading frame of 1?056?bp which encoded a putative protein of 351 amino acids.Further sequence analysis showed that it was a splicing variant of p44S10 gene family. The deduced protein encoding a regulatory particle of 26S proteasome has been highly conserved during evolution,suggesting the importance of this gene transcript in organism.

关 键 词：基因转录 克隆 序列分析 基因表达 遗传学 睾丸 

分 类 号：R394[医药卫生—医学遗传学]