含未甲基化CpG寡脱氧核苷酸对人外周血树突状细胞抗肿瘤作用的影响  

Antitumor effect of CpG ODN on human peripheral blood dendritic cells

作　　者：刘艳[1] 杨宁[1] 黄少萍[1] 刘方舟[1] 尹兰珍[1] 

机构地区：[1]东南大学基础医学院人体解剖与组织胚胎学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2004年第4期232-235,共4页Journal of Southeast University(Medical Science Edition)

基　　金：铁道部科技基金资助项目 (J99Z2 1 8)

摘　　要：目的 :研究含未甲基化CpG寡脱氧核苷酸 (CpGODN)对人外周血树突状细胞 (dendriticcells ,DC)分化、成熟及其抗肿瘤作用的影响。方法 :分离正常人外周血DC ,透射电镜观察CpGODN刺激组和未刺激组DC的超微结构 ,流式细胞仪分析其表面HLA DR、CD86的表达 ,MTT法检测其对同种异体混合淋巴细胞反应 (mixed lymphocytereactor ,MLR)中T细胞增殖的影响及调节T细胞杀伤肿瘤的能力。结果 :与未刺激组相比 ,CpGODN刺激组DC表面突起细、长且规则 ,粗面内质网增多 ,空泡减少甚至消失 ;同时DC表面HLA DR、CD86的表达上调 ,能明显促进MLR中T细胞的增殖 ,增强DC调节T细胞杀伤肿瘤活性。结论 :CpGODN可诱导人外周血DC分化成熟 。Objective To investigate the effect of CpG ODN on the differentiation,maturation and antitumor activity of human peripheral blood dendritic cellsDC.Methods DC was isolated from normal human peripheral blood. Then the ultrastructure characteristics of DC that incubated with and without CpG ODN were observed under transmisson electron microscope,and their expression of surface antigen HLA?DR,CD86 was analyzed by flow cytometry,and the effect on the proliferation of naive T cell in allogeneic mixed lymphocyte reactionMLR and antitumor experiment were detected by MTT test.Results Thin,long and regular dendrites with numerous rough endoplasmic reticulum and scanty vacuole were observed on the surface of GpG ODN stimalated DC,while it didn't occur in non-CpG ODN-stimulated DC. Meanwhile,CpG ODN up-regulated the expression of HLA?DR、CD86 on DC,improved significantly the proliferation of T cell in MLR and enhanced the antitumor activity of DC,superioring to non-CpG ODN-stimulated group.Conclusion CpG ODN may induce the differentiation and maturation of DC and increase the antitumor capacity of DC.

关 键 词：未甲基化 CPG 寡脱氧核苷酸 外周血 树突状细胞 肿瘤 免疗疗法 

分 类 号：R730.51[医药卫生—肿瘤]