CEA-HBD2重组基因在人结肠癌细胞中表达初步研究  

The test of expression of recombinant CEA-HBD2 in colon cancer HCT116 cell

作　　者：杜剑平[1] 张青[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥全军肿瘤中心,重庆400037

出　　处：《现代临床医学生物工程学杂志》2004年第3期203-205,共3页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然基金资助;项目资助编号 :30 1 70 4 0 4

摘　　要：目的　构建高表达人源化HBD2人结肠癌表达体系 .方法　构建人pLNCX2 -CEA信号肽—HBD2抗菌肽(成熟肽序列 )逆转录表达载体 ,DOTAP转染逆转录包装细胞 ,产生的含融合基因的侵袭性逆转录病毒 ,转染人结肠癌HCT116细胞 ,用免疫印迹法法鉴定转化细胞中HBD2蛋白的表达 .结果　正确构建了表达HBD2成熟肽的重组信号肽HBD2的人结肠癌表达体系 ,并出现正确的目的基因表达 .结论　此HBD2表达体系表达的特异性多肽经纯化后 。Objective To construt highly expression of HBD2 system in eucaryotic cell. Methods To construct recombinant retrovirus expression vector pLNCX2-CEA signal peptide-HBD2(mature peptide sequence),and then transfect the revirus vecter to PT67 cell by DOTAP,after screening and amplify single clone cell by G418,collecting the virus and tranfect the virus to eucaryotic cell HCT116 cell.The HCT116 cell has also been screening by G418 and get resistance cell clone, the expression of HBD2 was detected by western blotting. Conclusion The result indicate high level expression of HBD2 was obtained in HCT116 cell.

关 键 词：HBD2 CEA 融合基因 蛋白表达 

分 类 号：R735.35[医药卫生—肿瘤]