变形链球菌变链素粗提物活性的初步研究  

Preparation and antibacteria activity of crude mutacin from clinical isolate of Mutans streptococci

作　　者：李颂[1] 刘天佳[1] 付春华[1] 杨德琴[1] 庄姮[1] 杨锦波[1] 刘昭慧[1] 

机构地区：[1]四川大学华西口腔医学院,610041

出　　处：《实用口腔医学杂志》2004年第4期422-424,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金 (编号 30 1 71 0 1 3);国家教委博士点基金 (编号 0 1 0 61 0 33);安徽省教委基金资助

摘　　要：目的 :测定变链菌临床株变链素粗提物的抑菌活性和热稳定性 ,为对变链素的进一步研究提供基础。方法 :用氯仿抽提 ,对其进行抗菌活性检测和热稳定性检测。结果 :10ml液体的粗提物 ,可形成直径 19mm的抑菌环 ,表明粗提物具有抗菌活性 ;将粗提物加热到 80℃ ,抗菌活性可维持 12 0min ,表明其中含有热稳定性抗菌物质。结论 :变链素的粗提物能反映变链素的活性和性质 ,适合于大量临床株的研究。Objective:To obtain crude mutacin produced by clinical isolate of Mutans streptococci (Ms) and to test its antibacteria activity. Methods:The mutacin in supernatant of in vitro cultured clinical isolate of Ms was extracted by chloroform, the antibacteria activity and heat stability of the crude extract were tested by bacteria culture technique. Results:Crude extract of protein was obtained fom clinical isolate of Ms. 10 ml of the liquid extract produced a 19 mm inhibitory zone in cultrue dish, indicating its antibacteria activity. The activity could maintain in 80 ℃ for 120 min, indicating its heat stability. Conclusion: The crude extract can represent the antibacteria activity and heat stability of mutacin.

关 键 词：变形链球菌 变链素粗提物 热稳定性 抑菌活性 

分 类 号：R780.2[医药卫生—口腔医学]