Survivin对辐射损伤的影响及与caspase-9/6、3的关系  

Effects of survivin on radiation injury and its relation to Caspase-9/6,3 activity

作　　者：高洋[1] 罗成基[1] 黎海蒂[2] 郑业华[3] 

机构地区：[1]第三军医大学预防医学院全军复合伤研究所 [2]第三军医大学基础医学部生理学教研室,重庆400038 [3]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2004年第15期1350-1353,共4页Journal of Third Military Medical University

摘　　要：目的　观察survivin对辐射损伤的影响及与caspase 9 6、3的关系。方法　①将IEC 6细胞放置于 2 %O2 环境中低氧 8h后 ,复氧 2 4、48h ,采用Western blot检测胞内survivin ;②给予不同实验组IEC 6细胞 3 5Gy6 0 Co辐射照射 ,采用细胞流式PI法检测细胞凋亡率 ,并使用荧光免疫检测试剂盒检测各组caspase 9 6、3活性。结果　低氧预处理方法可诱导IEC 6细胞表达survivin ,Survivin可明显降低辐射诱导的细胞凋亡率 (P <0 .0 1) ,其对caspase 9 6活性无明显影响 (P >0 0 5 ) ,可使caspase 3活性有明显下降 (P <0 .0 1)。结论　低氧预处理方法诱导的抗凋亡蛋白survivin是通过抑制caspase 3的活性 。Objective To explore the effects of survivin on radiation injury and its relation to caspase 9/6,3 activity. Methods ① IEC 6 cells were treated under hypoxic environment for 8 h and followed by reoxygenation for 24 and 48 h, and then the expression of survivin was identified by Western blotting. ② After treatment with 35 Gy 60 Co , the apoptosis rate in different groups was detected with FACS and the caspase 9/6,3 activity was tested using the caspase 9/6,3 assay kit. Results Hypoxic preconditioning could induce the expression of survivin in IEC 6 cells and survivin could decrease the caspase 3 activity, resulting in reduced apoptosis rate induced by radiation. Conclusion Survivin induced by hypoxic preconditioning can inhibit the apoptosis induced by radiation by reducing the caspase 3 activity.

关 键 词：SURVIVIN 辐射 细胞凋亡 CASPASE 抗凋亡蛋白 

分 类 号：Q557.9[生物学—生物化学] R394.2[医药卫生—医学遗传学]