脂肪酸对糖尿病模型大鼠血磷脂脂肪谱的影响  

Effect of dietary fatty acids on fatty acids composition of serum phospholipids in diabetes mellitus model rats

作　　者：史继虹[1] 史虹莉[2] 方京冲[2] 郭红卫[1] 

机构地区：[1]复旦大学公共卫生学院营养与食品卫生学教研室,上海200032 [2]复旦大学附属华山医院糖尿病研究室

出　　处：《中国公共卫生》2004年第5期542-544,共3页Chinese Journal of Public Health

摘　　要：目的　观察富含n - 6和n - 3多不饱和脂肪酸 (PUFA)饲料对自发性 2型糖尿病模型OtsukaLong -E vansTokushimaFatty(OLETF)大鼠糖代谢及血清磷脂 (S -PL)中脂肪酸 (FA)组成的影响。 方法　将 2 0只 14周龄雄性OLETF大鼠随机分为 2组。两组大鼠饲料总能量相同 ,其中饱和脂肪酸 (SFA)、单不饱和脂肪酸 (MUFA)和PU FA的百分含量亦相同 ,但分别富含n - 6PUFA〔4 1 6mol/ (10 0mol·脂肪 )〕和n - 3PUFA〔2 0 4mol/ (10 0mol·脂肪 )〕 ,饮食干预 10周。第 2 4周时实施口服糖耐量试验 (OGTT)、胰岛素水平测定和S -PL中FA组成测定。结果　干预期间 ,两组大鼠总进食量及体重增长无显著性差异 ;干预后OGTT及胰岛素水平测定表明 ,n - 3组相对于n - 6组具有较好的胰岛素敏感性。n - 3组大鼠S -PL中 18∶1(n - 9)、MUFA总量、18∶2 (n - 6 )、各n - 3系PUPA百分含量较n- 6组高 ,n - 6PUFA总量、2 0∶4 (n - 6 )百分含量较低。结论　n - 3PUFA的摄入相对于n - 6PUFA对OLETF大鼠的糖代谢有益 ,OLETF大鼠S -PL的FA组成受到饲料中FA的影响 ,但并不是其直接反应。Objective To investigate the influence of dietary n-3 compared with n-6 PUFA on the FA composition of S-PL and glucose metabolism in OLETF rats,a model of spontaneous type 2 diabetes mellitus.Methods 20 male OLETF rats of 14-week age were divided into 2 groups randomly.Isocaloric diets contained either n-6PUFA 〔41.6 mol/(100?mol·fats)〕 or n-3PUFA 〔20.4?mol/(100?mol·fats)〕 but had identical contents of SFA,MUFA and PUFA.An OGTT and measurement of insulin level was performed at 24 weeks of age after 10-week treatment.The FA composition of S-PL in the two groups of rats was observed as well.Results The food intake and bodyweight gain were comparable in n-6 and n-3 groups.The results of OGTT and measurement of serum insulin level showed that the n-3 diet had a more beneficial effect on insulin sensitivity than the n-6 diet.The S-PL in the rats of the n-3 diet had higher contents of 18:1(n-9),total MUFAs,18:2(n-6),n-3 series PUFAs and lower contents of 20:4(n-6),total n-6PUFAs than in the n-6 diet group.Conclusion The n-3 diet had a more beneficial effect on glucose metabolism than the n-6 diet.The FA composition of S-PL was influenced by the dietary fats but not a direct reflection of dietary FA composition in OLETF rats.

关 键 词：膳食脂肪 多不饱和脂肪酸 血清磷脂脂肪酸 糖代谢 

分 类 号：R587.1[医药卫生—内分泌]