神经母细胞瘤骨髓转移细胞体外原代培养技术的实验研究  

In vitro culture of bone marrow neuroblastoma metastatsis

作　　者：李爱敏[1] 张锦华[1] 王弘[1] 姜俊[1] 宓真[1] 张成红[2] 陈世洁[1] 陈誉华[2] 于秉治[3] 

机构地区：[1]中国医科大学第二附属医院血液研究室,沈阳110004 [2]中国医科大学细胞生物研究室 [3]中国医科大学药理教研室

出　　处：《中华小儿外科杂志》2004年第3期270-273,共4页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金 ( 3 9470 73 9) ;辽宁省科学技术基金重大项目 ( 2 0 0 110 10 0 1)

摘　　要：目的　根据神经母细胞瘤 (neuroblastoma,NB)易发生早期骨髓转移的特点 ,建立NB骨髓转移细胞体外原代培养的实验技术。方法　对 2 7例伴骨髓转移的NB患者进行NB骨髓转移细胞体外原代培养 ,并与已建立的SMS KCNR和SH SY5Y细胞系进行比较 ,在倒置相差显微镜下观察细胞生长情况及形态学特点 ,同时应用单克隆抗体F3系列检测原代培养的NB骨髓转移细胞。结果　在 2 7例NB骨髓转移细胞体外原代培养中 ,培养成功 9例 ,其细胞生长情况及形态学特点与SMS KCNR和SH SY5Y细胞系基本一致 ,单克隆抗体F3系列检测均阳性。结论　NB骨髓转移细胞体外原代培养技术的成功建立为NB体外诱导逆转及多药耐药等复杂机制研究提供更接近体内生物学性状的细胞来源。Objective To establish metastatic neuroblastoma cell lines from bone marrow.Methods Metastatic neuroblastoma cells from bone marrow of 27 patients were cultured in vitro. The morphological features were examined and compared with the human SMS-KCNR and SH-SY5Y cell lines. Monoclonal F3 antibody was used to detect and confirm the cultured neuroblastoma cells.Results Of 27 metastatic neuroblastoma cell cultures,9 were successful and their morphological features were consistent with those of the human SMS-KCNR and SH-SY5Y cell lines. F3 antibody in all 9 cases was positive.Conclusion The successful establishment of metastatic neuroblastoma cell lines from bone marrow in vitro could provide valuable tool in understanding tumor differentiation and multiple drug resistance of NB.

关 键 词：神经母细胞瘤 骨髓转移细胞 体外原代培养技术 肿瘤 NB 瘤细胞 

分 类 号：R73-3[医药卫生—肿瘤]