全反式维甲酸诱导大鼠胚胎马蹄内翻足模型  

Study on rat fetuses talipes equinovarus model induced by all-trans retinoic acid

作　　者：周海涛[1] 孙开来[1] 李增钢 赵彦艳[1] 李岭[1] 吉士俊[3] 马瑞雪[3] 尚超[1] 李春义[1] 富伟能[1] 

机构地区：[1]中国医科大学医学遗传教研室,沈阳110001 [2]国家新药安全评价中心 [3]中国医科大学附属第二医院小儿骨科

出　　处：《中国公共卫生》2004年第4期413-415,共3页Chinese Journal of Public Health

基　　金：国家 973计划资助项目 (0 0 1CB51 0 30 1 )

摘　　要：目的　利用全反式维甲酸 (ATRA)诱导Wistar大鼠 ,建立马蹄内翻足动物模型 ,分析ATRA最适诱导剂量。方法　将Wistar孕鼠随机分为 4个用药组及对照组 ,孕 10d分别按 12 0 ,130 ,135 ,14 0mg/(kg·bw)不同剂量经口给予ATRA矿物油混悬液和纯矿物油。孕 2 1d剖检胎鼠 ,并对足踝部切片观察。结果　ATRA可诱导Wistar大鼠胚胎发生马蹄内翻足样畸形及其它畸形 ,发生率随剂量的增加而增高 ;单纯马蹄内翻足样畸形的发生率 135mg/(kg·bw)组为最高 ,达 2 9 2 7%。结论　 135mg/(kg·bw)ATRA是诱导马蹄内翻足样畸形的理想剂量。Objective To establish rat fetuses talipes equinovarus model induced by all-trans retinoic acid and investigate the optimum dosage of all-trans retinoic acid.Methods Wistar rats were distributed randomly into a control group and 4 assay groups.On day 10 of pregnancy,variable dose of 120,130,135 and 140mg/(kg·bw) all-trans retinoic acid suspended in mineral oil was given intragestrically to the rats of each assay group and equivalent volume of mineral oil of the 140mg/(kg·bw) group was given to the control rats.Fetuses were delivered and examined under stereomicroscope on day 21 of pregnant.The hindlimbs were removed, processed,sliced up as serial sections in three planes of foot,analysed under a light microscope.Results Deformity of talipes equinovarus and other external malfomlations were induced by all-trans retinoic acid on Wistar rat fetuses.The incidence of simplex deformity of talipes equinovarus was the highest in the 135mg/(kg·bw) assay group.Conclusion 135mg/(kg·bw) all-trans retinoic acid was the optimal dosage for inducing deformity of talipes equinovarus.

关 键 词：马蹄内翻足 维甲酸 动物模型 

分 类 号：R394-33[医药卫生—医学遗传学]