Lack of evidence for leukocyte maternal microchimerism in primary biliary cirrhosis  

作　　者：Kenichi Nomura Yoshio Sumida Takaharu Yoh Atsuhiro Morita Yosuke Matsumoto SawakoTaji NaohisaYoshida MasahitoMinami YoshitoItoh ShigeoHoriike Keisho Kataoka Masafumi Taniwaki Takeshi Okanoue 

机构地区：[1]Molecular Hematology and Oncology,Kyoto Prefectural University of Medicine Graduate School of Medical Science,Kyoto 602-0841,Japan [2]Department of Internal Medicine,National Nara Hospital,Nara 630-8305,Japan [3]Department of Internal Medicine,Aiseikai Yamashina Hospital,Kyoto 607-8086,Japan [4]Ayabe city Hospital,Kyoto 623-0011,Japan [5]Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine Graduate School of Medical Science,Kyoto 602-0841,Japan [6]Clinical Molecular Genetics and Laboratory Medicine,Kyoto Prefectural University of Medicine Graduate School of Medical Science,Kyoto 602-0841,Japan

出　　处：《World Journal of Gastroenterology》2004年第16期2415-2416,共2页世界胃肠病学杂志（英文版）

基　　金：Supported by the grants of the Ministry of Education,Science,Sports,and Culture of Japan,No.15790497

摘　　要：AIM:It is reasonable to assume that microchimerism could also be involved in the induction of primary biliary cirrhosis (PBC).However,previous reports investigated only fetus-microchimerism in women patients.Maternal microchimerism has not been investigated until now. The current study aimed to clear either maternal microchimerism was involved in the pathogenesis of PBC or not. METHODS:We used fluorescence in situ hybridization on paraffin-embedded tissue (We called“Tissue-FiSH”.) to determine whether maternal cells infiltrated in male patients who were diagnosed as having PBC.Tissue-FiSH was performed by using both X and Y specific probes on the biopsy liver sample of 3 male PBC patients. RESULTS:Infiltrating lymphocytes demonstrated both X and Y signals in all 3 male patients. CONCLUSION:Maternal microchimerism dose not play a significant role in PBC.PBC may not relate to fetus and maternal microchimerism.It is reasonable to assume that microchimerism could also be involved in the induction of primary biliary cirrhosis (PBC). However, previous reports investigated only fetus-microchimerism in women patients. Maternal microchimerism has not been investigated until now.The current study aimed to clear either maternal microchimerism was involved in the pathogenesis of PBC or not.METHODS: We used fluorescence in situ hybridization on paraffin-embedded tissue (We called 'Tissue-FiSH'.) to determine whether maternal cells infiltrated in male patients who were diagnosed as having PBC. Tissue-FiSH was performed by using both X and Y specific probes on the biopsy liver sample of 3 male PBC patients.RESULTS: Infiltrating lymphocytes demonstrated both X and Y signals in all 3 male patients.CONCLUSION: Maternal microchimerism dose not play asignificant role in PBC. PBC may not relate to fetus and maternal microchimerism.

关 键 词：CHIMERA Chromosomes  Human  X Chromosomes  Human  Y Female Humans In Situ Hybridization  Fluorescence Leukocytes Liver Liver Cirrhosis  Biliary LYMPHOCYTES Male Research Support  Non-U.S. Gov't 

分 类 号：R575.2[医药卫生—消化系统]