RhoA、RhoC及其效应分子ROCK-1的表达与卵巢癌细胞系恶性行为相关性的体外研究  

Correlation of expression of RhoA,RhoC and their effector ROCK-1 with malignan t phenotype of ovarian cancer cells in vitro

作　　者：韩志强[1] 张阿丽[1] 吴明富[1] 刘玉兰[1] 陈刚[1] 李辅军[1] 高庆蕾[1] 廖国宁[1] 卢运萍[1] 王世宣[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中华肿瘤杂志》2004年第7期385-388,共4页Chinese Journal of Oncology

基　　金：国家杰出青年基金项目 (3 0 0 2 5 0 17) ;国家重点基础研究发展规划项目 (2 0 0 2CB5 13 10 0 )

摘　　要：目的　研究RhoA、RhoC及其效应分子ROCK 1在卵巢癌细胞中的表达水平 ,探讨其与卵巢癌转移的相关性。方法　逆转录聚合酶链反应 (RT PCR)检测RhoA、RhoC及ROCK 1在SW6 2 6、Skov 3、A2 780和Caov 34种卵巢癌细胞中mRNA的表达水平 ;Westernblot法检测RhoA和ROCK 1蛋白质的表达情况 ;Boyden小室体外侵袭试验测定 4种卵巢癌细胞的体外迁移与侵袭能力。结果　RhoA、RhoC和ROCK 1在 4种卵巢癌细胞中呈不同程度表达 ,其中仅RhoC的表达水平与癌细胞侵袭力和迁移能力呈正相关 (r=0 .95 ,P <0 .0 1)。RhoA在转录水平和蛋白水平中呈不同步变化 ,且RhoA和RhoC的表达与ROCK 1的表达无相关性。 4种卵巢癌细胞的体外侵袭和迁移能力相一致 ,其中SW6 2 6最强 ,Caov 3最弱 ,Skov 3和A2 780居中。结论　RhoC的表达与卵巢癌细胞的体外侵袭和迁移能力相关 ,可能作为一个独立因素在卵巢癌的转移过程中起作用 ,有望成为阻断卵巢癌转移的新靶点。Objective To investigate the expression of RhoA,RhoC and their eff ector ROCK-1 in four ovarian cancer cell lines in vitro and their correlation w ith invasiveness. Methods Expression of RhoA,RhoC and ROCK-1 mRNA and protein in four ovarian cancer cell lines SW626,Skov-3,A2780 and Caov-3 was detecte d by RT-PCR and Western blot assay. Invasion assay was done in Boyden chamber. Results The expression levels of RhoA,RhoC and ROCK-1 mRNA and protein varied in the four different cell lines examined. The expression level of RhoC,but not R hoA and ROCK-1,was significantly correlated with the invasive capability of th e se cells in vitro ( r =0.95, P <0.01). Expression of RhoA at the level of t ranscrip tion was not correlated with that at the translation level. The expression of Rh oA and RhoC did not correlate with that of ROCK-1. Conclusion Expression level of RhoC may serve as an independent parameter in evaluating metastasis and beco me a new target in inhibiting ovarian cancer metastasis.

关 键 词：RHOA RHOC 效应分子 ROCK-1 基因表达 卵巢癌 癌细胞 恶性行为 肿瘤转移 

分 类 号：R737.31[医药卫生—肿瘤]