黄芪多糖的分子量分布  

Molecular Mass Distribution of Astragalus Polysaccharides

作　　者：闫巧娟[1] 韩鲁佳[1] 江正强[2] 皇才进[1] 

机构地区：[1]中国农业大学工学院,北京100083 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2004年第8期27-30,共4页Food Science

基　　金：国家"十五"攻关子课题(2002BA514A-12-3)

摘　　要：本文通过分级醇沉、分步醇沉和超滤方法研究黄芪多糖的分子量分布。结果表明:分级醇沉浓度对应的得率随醇浓度的升高而呈增加趋势;糖的含量变化起伏较大,醇浓度为60%时所得多糖含量比较低,醇浓度为30%和70%时所得多糖含量较高;分步醇沉的醇浓度为10%时沉淀下来的多糖所占比例最大,醇浓度达到80%时大部分多糖都可以沉淀下来;而所得多糖含量除90%醇浓度时较低外,其余相差不大;超滤后黄芪多糖大部分分布在离心沉淀和截留分子量150千道尔顿(150kDa)以上部分,两者占了57.6%;其含量除3kDa以下和6kDa至50kDa部分较低外,其它部分含量相差不大。黄芪多糖的分布很不均匀,大分子量和小分子量的多糖较多,而3kDa至150kDa这一段的多糖只占总多糖的13.2%。In the study, molecular mass distribution of Astragalus Polysaccharides (APS) was investigated by ethanolprecipitation (step and stepwise) and ultrafiltration. The results indicated: For step ethanol precipitation, yields of APS wereincreased with the increase of ethanol concentration. There were high differences in the saccharides content of samples, the lowcontent was observed when using 60% ethanol, but, high contents were obtained using 30% and 70% respectively. For stepwiseethanol precipitation, APS ratio was highest at ethanol concentration of 10%, while most APS might be precipitated whenethanol concentration was up to 80%. APS content was very low when using 90% ethanol, and the rest contents were notapparently different under ethanol concentrations ranging from 10% to 80%. After ultrafiltration, most APS (57.6%) weredistributed in the centrifuged pellets of more than 150kDa fraction. There was not much difference in the saccharides contentsexcept for those of less than 3kDa and 6～50kDa fractions. Both high and low molecular masses of APS were of highpolysaccharides, however the saccharide content of 3～150kDa APS was only 13.2%. Therefore, molecular mass distributionwas very uneven in APS.

关 键 词：黄芪多糖 醇沉 超滤 分子量分布 醇浓度 

分 类 号：TS201.2[轻工技术与工程—食品科学]