电针对佐剂性关节炎大鼠病灶局部皮肤组织IL-1β、IL-1ra mRNA表达的影响  

Effect of Electroacupuncture on Expression of IL-1β mRNA and IL-1ra mRNA in the Skin Tissue of the Pain Focus in Adjunvant Arthritis Rats

作　　者：李熳[1] 王丽娜[1] 易春霞[1] 孟宪芳[1] 曾青[1] 张静[1] 李玲俐[1] 施静[1] 关新民[1] 

机构地区：[1]华中科技大学同济医学院神经生物学系,武汉430030

出　　处：《针刺研究》2004年第3期187-191,共5页Acupuncture Research

基　　金：国家自然科学基金重大研究计划资助项目 (90 2 0 90 0 9) ;湖北省自然科学基金资助项目 (2 0 0 2AB1 2 4 ) ;华中科技大学医工;医理学科交叉科研基金资助项目。

摘　　要：目的 :观察电针对单侧佐剂性关节炎大鼠的疼痛试验评分、炎症灶局部病理反应 ,以及病灶局部皮肤组织IL 1β、IL 1ramRNA表达的影响。方法 :通过将完全弗氏佐剂 50 μL注入SD大鼠左后肢外踝关节皮下制造单侧佐剂性关节炎疼痛模型 ,电针“环跳”穴、“阳陵泉”穴 (刺激参数为0 .5～ 1.5V ,4～ 16Hz,3 0min) ,应用跖、背屈关节评分连续观察大鼠 7d内的痛反应曲线 ,应用HE组织化学染色方法观察病灶局部皮肤组织炎性病理反应 ,应用RT PCR技术检测IL 1β、IL 1ramRNA表达情况。结果 :电针可明显减少炎症痛大鼠的疼痛评分 ,抑制佐剂性关节炎大鼠病灶局部炎性病理反应 ,并抑制病灶局部皮肤组织IL 1βmRNA表达 ,促进IL 1ramRNA表达 ,使IL 1ra/IL 1β比值上升 (P <0 .0 1)。结论 :电针可能通过调节炎症灶局部致炎细胞因子及抗炎细胞因子之间的平衡 ,从根本上解除局部病灶免疫细胞的激活状态 ,达到扶正祛邪、平衡阴阳的调控作用 ,从外周途径缓解疼痛。Objective: To elucidate the effect of electroacupuncture (EA) on arthritic flexion pain scores, pathological reaction and IL-1β mRNA and IL-1ra mRNA expression of the skin tissue of the pain focus in adjunvant monoarthritis rats. Methods: 39 SD rats were randomized into control group (n=13), inflammation group (n=13) and EA group (n=13). Arthritis model was established by subcutaneously injection of 50 μL complete Freund’s adjuvant (CFA) into unilateral rat’s hind-paw. EA (0.5～1.5 V, 4～16 Hz) was applied to “Huantiao”(GB 30) and “Yanglingquan” (GB 34) for 30 minfollowing injection of CFA. Arthritic flexion pain scores were given everyday and continuously for 7 days. At the end of the experiments, the focal skin tissue was taken to be stained by hematoxylin eosin (HE) method for detecting the inflammatory cell number (in 100×100 μm2 section), and the expression of interleulin (IL)-1β mRNA and IL-1 receptor antagonist (IL-1ra) mRNA was detected by RT-PCR. Results: ① After EA treatment, compared with inflammation group, the arthritic flexion pain scores of EA group reduced significantly (P<0.01); ② The inflammatory cells of the focus induced by CFA in EA group (45.71±5.92) were significantly fewer than those of inflammation group (95.66±6.53, P<0.01); ③ The IL-1β mRNA expression of EA group was significantly lower than that of inflammation group (P<0.01), while IL-1ra mRNA expression of EA group considerably higher than that of inflammation group (P<0.01), and IL-1ra/IL-1β of EA group (1.723± 0.049) was also significantly higher than that of inflammation group (0.948±0.034, P<0.01). Conclusion: EA can regulate the balance between IL-1β and IL-1ra to inhibit the activation of the immune cells in the pain focus, thus exert the effect of analgesia.

关 键 词：局部 病灶 IL-1RA MRNA表达 IL-1Β 佐剂性关节炎 大鼠 方法观 

分 类 号：R735[医药卫生—肿瘤] R743[医药卫生—临床医学]