转4-1BBL基因的小鼠肝癌细胞瘤苗体内抗癌作用的研究  

In vivo anticancer effects induced by gene transfer of 4-1BBL into murine hepatocellular carcinoma cell line Hepa1-6

作　　者：刘承利[1] 窦科峰[2] 朱帮福[3] 臧晓霞[4] 陈苏民[3] 

机构地区：[1]空军总医院肝胆外科,北京100036 [2]第四军医大学西京医院肝胆外科 [3]第四军医大学基础部生化教研室 [4]第四军医大学口腔医学院牙体科

出　　处：《中华普通外科杂志》2004年第6期352-354,共3页Chinese Journal of General Surgery

摘　　要：目的研究转 4 1BBL基因的小鼠肝癌细胞疫苗在小鼠体内诱导的抗癌作用。方法采用脂质体介导法将真核表达质粒 pcDNA3.1 (+) m4 1BBL导入小鼠肝癌细胞Hepa1 6 ,经G4 1 8筛选后获得稳定高表达克隆 ,以丝裂霉素C(mitomycin ,MMC)处理后 ,制成癌细胞瘤苗 (tumorcellvac cine ,TCV) ,观察其对不同动物模型的体内免疫保护作用和免疫治疗作用。结果TCV 4 1BBL能刺激小鼠产生针对其野生型肝癌细胞的免疫保护作用 ,可以使免疫后的小鼠长期保持无癌状态 ;对早期癌模型有较强的治疗作用 ,但对晚期癌模型未见疗效。结论转 4Objective To study the effects of 4 1BBL on antitumor immunity induced in vivo by murine 4 1BBL gene transfected hepatocellular carcinoma cell line Hepa1 6. Methods Eukaryotic expression vector pcDNA3 1(+) m4 1BBL was transfered into murine hepatocellular carcinoma cell line Hepa1 6 via lipofectamine method. The transfected cells were selected in RPMI1640 containing G418(400～800 μg/ml) and termed as Hepa1 6 m4 1BBL.The TCV m4 1BBL was obtained by treating with mitomycin (MMC). Three models (immunological model, early model, and later model) were established for the study of anticancer effects of TCV m4 1BBL. Results The syngeneic mice were completely protected and could survive free from tumor for a long period by inoculation with TCV 4 1BBL. In early models, TCV 4 1BBL showed strong immunotherapy effects( P <0 05). But in later models, no obvious antitumor effects were observed ( P >0 05). Conclusion The antitumor effect against syngeneic murine hepatocellular carcinoma cell line in vivo is significantly enhanced by the treatment with TCV 4-1BBL.

关 键 词：转4-1BBL基因 小鼠 肝癌 抗癌作用 疫苗 免疫治疗 

分 类 号：R735.7[医药卫生—肿瘤]