四氯化碳和猪血清肝纤维化模型组织病理比较  

Pathological comparison of hepatic-fibrosis models induced by porcine serum and carbon tetrachloride in rats

作　　者：刘秀英[1] 胡怡秀[1] 胡余明[1] 丘丰[1] 周月婵[1] 臧雪冰[1] 聂焱[1] 

机构地区：[1]湖南省疾病预防控制中心毒理科,湖南省长沙市410005

出　　处：《世界华人消化杂志》2004年第8期1875-1879,共5页World Chinese Journal of Digestology

基　　金：湖南省卫生厅科研基金资助项目;No.00080~~

摘　　要：目的:比较四氯化碳和猪血清染毒诱导的大鼠肝纤维化模型肝组织病理表现的异同. 方法:四氯化碳组、猪血清组大鼠分别于每周一、四腹腔注射.400 mL/L CCl4(2 mL/kg)、猪血清0.5 mL/鼠,连续7 wk.实验末取肝组织,常规切片,HE染色后光镜下观察肝组织病理改变;Masson三重染色、James染色后光镜下分别观察胶原纤维、网状纤维的增生情况,并采用病理图像系统进行半定量分析;另取部分肝组织,常规处理后电镜下观察超微结构变化. 结果:猪血清组肝组织纤维间隔纤细,包含明显的胶原沉积,间质细胞成分多,胶原沉积并不弥散,仅限于纤维间隔,偶见细胞损伤.四氯化碳组纤维间隔粗大,弥散分布,纤维间隔内细胞成分多;肝细胞损伤严重且广泛,脂肪变性明显.病理图像分析系统半定量分析显示四氯化碳组胶原纤维和网状纤维面密度、数密度、周密度均显著高于猪血清组(P<0.05或P<0.001). 结论:四氯化碳诱导的肝纤维化程度较猪血清严重.AIM: To compare liver pathological changes between two different hepatic-fibrosis models induced by porcine serum and carbon tetrachloride in rats. METHODS: Hepatic fibrosis was induced in rats by biweekly intraperitoneal injection of porcine serum (0.5 mL) or 400 mL/L carbon tetrachloride (2 mL/kg) for 7 weeks. Five rats were randomly selected from the survivals in each group. Liver tissue was removed, fixed, sliced and stained with hema-toxylin and esosin for routine light-microscopy, Masson trichrome for collagen, Jame's double ammoniated siver solution for reticulin, and then subjected to semiquantitative evaluation by pathological image analyzer. Another portion of liver tissue was prepared for electron microscopy. RESULTS: Liver tissue in rats receiving pig serum displayed narrow fibrotic sepatae including obvious collagen deposition and more mesenchymal constituents, and collagen was confined to the septae. Hepatocellular injury is rare. In contrast, in the livers from rats treated with CCl4, the fibrotic septae were coarse, more cellular and diffuse. The parenchymal damage was more severe and extensive. The fatty metamorphosis of hepatocytes was evident. Semiquantitative analyses suggested that quantity of collagenic fibers and reticular fibers in CCl4 group were significantly higher than those in porcine serum group. CONCLUSION: Hepatic fibrosis in rats induced by CCl4 is more severe than that induced by porcine serum.

关 键 词：四氯化碳 肝纤维化模型 肝组织病理 猪血清 严重 光镜 胶原 结论 图像系统 情况 

分 类 号：R575.2[医药卫生—消化系统]