营养干预对内毒素血症致营养不良雌性大鼠青春发动及胰岛素样生长因子1轴的影响(英文)  

Effect of a high protein and high caloric dieton the onset of puberty and insulin growthfactor-1 (IGF-1) axis in female rats withmalnutrition induced by endotoxemia

作　　者：张淼[1] 杜敏联[1] 马华梅[1] 赖峰[1] 

机构地区：[1]中山大学附属第一医院儿科,广东广州510089

出　　处：《中国当代儿科杂志》2004年第5期353-359,共7页Chinese Journal of Contemporary Pediatrics

摘　　要：目的　慢性营养不良恢复期可能会发生青春期发动提前 ,本研究探讨恢复期营养干预对内毒素血症致致营养不良幼年雌性大鼠青春发动及IGF 1轴的影响。方法　体重匹配的同龄同窝幼年雌鼠 ,随机分至对照组 (C组 )及LPS处理组 (L组 ) (均n =8)。实验 1,L1组于生后 7- 13d隔日注射内毒素脂多糖 (LPS)共 4次 ,模拟反复感染 ;实验 2 ,L2组于生后 14～ 2 0d同样给予LPS。对照组与相应L组同时注射 0 .1mlPBS ,并予正常饮食。恢复期L1及L2组予高蛋白高热卡饲料喂养至体重赶上相应的C1及C2组。观察雌鼠的阴道口开放 (VO)、第 1个动情 (E)日龄及动情后期 (M期 )卵巢黄体的形成 ;血浆TNFα、IGF 1及IGFBP - 3水平的变化。结果　①L1组VO及E日龄均与C1组相似 (33.13± 2 .90dvs 33.5 0± 2 .6 7d ;37.13± 2 .5 9dvs 35 .88± 1.81d ,P >0 .0 5 ) ;两组M期卵巢黄体数无统计学差异 (3.75± 1.6 7vs 3.13± 1.13,P >0 .0 5 )。②L1组内毒素血症期末血TNF α水平高于C1组 (1.13± 0 .5 7U/mlvs 0 .71± 0 .18U/ml,P <0 .0 5 ) ;而血IGF 1水平则低于C1组 (2 0 1.6 3±2 8.31ng/mlvs 2 5 0 .91± 35 .0 7ng/ml,P <0 .0 5 )。恢复早期L1组血IGF 1水平回升并高于C1组 (2 89.2 0±39.0 7ng/mlvs 2 4 9.88± 33.5 0ng/ml,P <0 .Objective An early onset of puberty may occur during convalescence from chronic malnutrition in children. This study aims to explore the effect of a high protein and high caloric diet during convalescence on the onset of puberty in female rats with malnutrition induced by recurrent endotoxemia, and to investigate the change of IGF-1 axis during the period. Methods This study was done using the randomized paired design and consisted of two experiments with newborn SD rats. Two female rats from the same litter and matched in weight were randomized to a control group (C group) or a lipopolysaccharide (LPS) treated group (L group) (both n=8). In Experiment one, during 7-13 days of age, rats in the L1 group were given 4 intraperitoneal injections of LPS every other day to duplicate recurrent infection. In Experiment two, during 14-20 days of age, rats in the L2 group were given LPS every other day in the same dose as L1 group. Rats in the C groups were given intraperitoneal injections of 0.1 ml PBS at the same time as the corresponding L group and were fed with a regular diet. During convalescence, rats in the L1 and L2 group were given high protein and high caloric diets (containing 30% casein and 10% fat) until their weights caught up with that of the matched rats in the C1 or C2 group. The age of vaginal opening (VO) and first estrus were observed, and the number of corpora lutea of their ovaries during metestrus (M) were examined. The concentrations of plasma IGF-1, IGF binding protein-3 (IGFBP-3) and TNF-α were measured at ages of 14, 17 and 28 days. Results 1) There were no significant differences between the L1 and C1 groups in the average age of VO and first estrus. The number of corpora lutea were similar in both groups. 2) On the 14 th day of age, the plasma TNF-α concentration of rats in the L1 group was higher than that of the C1 group ( 1.13± 0.57 U/ml vs 0.71± 0.18 U/ml, P< 0.05), and plasma IGF-1 concentration of rats in the L1 group was lower than that of the C1 group ( 201.63± 28

关 键 词：青春期 内毒素血症 营养 胰岛素样生长因子1 大鼠 

分 类 号：R151.3[医药卫生—营养与食品卫生学]