转基因大米与亲本大米猪回肠蛋白质及氨基酸消化率的比较研究  

THE COMPARISON OF ILEAL DIGESTIBILITY OF PROTEIN AND AMINO ACIDS IN GM RICE AND PARENTAL RICE

作　　者：韩军花[1] 杨月欣[1] 门建华[1] 边立华[1] 杨晓光[1] 朱元招[2] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]农业部饲料工业中心,北京100094

出　　处：《营养学报》2004年第5期362-365,共4页Acta Nutrimenta Sinica

基　　金：科技部国家转基因植物研究与产业化专项(No.JOO-C-003);法国达能膳食营养与宣教基金(No.DIC2002-02)

摘　　要：目的:比较表达豇豆胰蛋白酶抑制剂基因大米(转SCK基因大米)和亲本大米蛋白质和氨基酸消化率的差异,并探讨其生物利用率的异同。方法:选用猪为实验动物,通过外科手术法在回肠末端放置T型瘘管,分别喂饲转SCK基因大米和亲本大米,收集动物回肠末端消化产物,分析和计算两种大米蛋白质和氨基酸的消化率。结果:转SCK基因大米与亲本大米中蛋白质的表观和真消化率相似;氨基酸消化率测定结果显示,除亲本大米中赖氨酸的表观消化率与SCK基因大米相比有差异外,其余氨基酸的表观和真消化率均无显著性差异。结论:外源性基因的插入未改变大米中蛋白质和氨基酸的消化率,在蛋白质和氨基酸消化率方面基本满足“实质等同性”的要求。Objective: To compare the digestibility of protein and amino acids in parental rice and rice genetically modified with cowpeas trypsin inhibitor. Methods: Six experimental swine were surgically fitted with simple T-cannula at the terminal ileum, and fed with parental rice and rice genetically modified with cowpeas trypsin inhibitor alternately. The ileum digesta were collected for determination of apparent and true digestibility of protein and amino acids. Results: The apparent and true digestibility of protein is similar in these two types of rice. Except the apparent digestibility of lysine, there are no statistical significances in the apparent and true digestibility of other 17 amino acids. Conclusion: The digestibility of amino acids is not changed by the insert of foreign gene, so it can meet the request of 'substantial equivalence' in protein and amino acids digestibility.

关 键 词：氨基酸消化率 亲本 SCK基因 真消化率 蛋白质 豇豆胰蛋白酶抑制剂基因 回肠末端 外源性基因 转基因大米 改变 

分 类 号：R151.2[医药卫生—营养与食品卫生学]