H1启动子siRNA载体的构建及应用  

Construction and application of siRNA vector with H1 promotor

作　　者：刘萱[1] 李楚芳[1] 李平[1] 曹诚军 

机构地区：[1]事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2004年第5期444-446,共3页Letters in Biotechnology

摘　　要：利用双链RNA(dsRNA)调控基因表达已经成为研究基因功能的有力工具。用人H1启动子构建了pBS/H1PS小干扰RNA(siRNA)表达载体,用于在哺乳动物细胞中产生特异性dsRNA转录产物。通过对293细胞中的PSMA7分子进行表达抑制,证明该siRNA载体能够有效产生针对靶基因的RNA干扰(RNAi)效应。The use of double-stranded RNA (dsRNA) to manipulate gene expression is evolving into an increasingly powerful tool to study gene function in organisms. The pBS/H1PS small interference RNA vector using human H1 promotor to produce specific dsRNA transcription in mammalian cells was constructed. The successful inhabitation of PSMA7 in 293 cells through pBS/H1PS vector demonstrate that this siRNA vector could induce effective RNA interference.

关 键 词：小干扰RNA 启动子 293细胞 PSMA 靶基因 载体 表达 双链RNA 哺乳动物细胞 RNA干扰 

分 类 号：Q756[生物学—分子生物学] R737.25[医药卫生—肿瘤]