人重组骨形成蛋白-2诱导NIH3T3细胞向成骨细胞表型转化  

Recombinant Human Bone Morphogenetic Protein-2 Transform NIH3T3 Fibroblast to Osteo-like Cells

作　　者：王军琳[1] 刘源[1] 金岩[1] 王新文[1] 李媛[1] 

机构地区：[1]第四军医大学口腔医学院组织病理学教研室,陕西西安710032

出　　处：《华西口腔医学杂志》2004年第5期420-422,共3页West China Journal of Stomatology

摘　　要：目的 探讨人重组骨形成蛋白-2(rhBMP-2)对NIH3T3成纤维细胞生物学行为的影响。方法 培养NIH3T3细胞,加入外源性rhBMP-2,用生长曲线、噻唑蓝(MTT)比色、流式细胞仪(FCM)分析、BrdU检测观察细胞的增殖和DNA合成,并检测碱性磷酸酶(AIP)活性和骨钙素(OC)含量观察细胞是否有成骨趋向。结果 加药组细胞的增殖活性下降、DNA合成变慢,ALP活性和OC含量均增高。结论 rhBMP-2使NIH3T3细胞增殖活性变弱,表现成骨细胞表型。Objective To examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NTH3T3 cells. Methods The NTH3T3 cells were cultured in DMEM with 50 μg/L rhBMP-2. The proliferation ability, alkaline phos-phatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated. Results The cells showed decreased proliferation ability but enhanced ALP activity and OC quantity. Conclusion 50μg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells.

关 键 词：人重组骨形成蛋白-2 RHBMP-2 NIH3T3细胞增殖 成骨细胞 表型转化 OC DNA合成 生长曲线 碱性磷酸酶 成纤维细胞 

分 类 号：R780.2[医药卫生—口腔医学]