氢化可的松高转化菌株的选育及其发酵条件  

BREEDING OF HIGH HYDROCORTISONE TRANSFORMING STRAINS AND THEIR OPTIMAL FERMENTATION CONDITIONS

作　　者：王敏[1] 郭亚文[1] 卢文玉[1] 包慧珂[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《应用与环境生物学报》2004年第5期663-666,共4页Chinese Journal of Applied and Environmental Biology

基　　金：天津市重大科技攻关项目 (0 2 31 80 2 1 1 );教育部科技研究重点项目(天津 0 1 0 0 4 )~~

摘　　要：利用酮康唑抗性突变选育氢化可的松高转化菌株 ,即将经过紫外线诱变处理的新月弯孢霉原生质体倾注于含有酮康唑最小抑制浓度 (10 μmol/L)的培养基平板上 ,获得了 136株酮康唑抗性突变株 ,氢化可的松转化率高于出发菌株的有 14株 .其中抗性突变株KA - 91,其氢化可的松转化率为出发菌株的 1.4 2倍 ,达到 6 8.0 5 % .研究了菌株的发酵性能 ,优化了培养基组成和发酵工艺条件 ,氢化可的松转化率达到 73.6 % .图 7表 3参A method of ketoconazole resistance screening was applied to breed mutants with high hydrocortisone transformation. The protoplast of Curvularia lunata, treated with UV light were regenerated on agar plates containing 10 μmol/L ketoconazole and 136 ketoconazole resistance mutants were obtained. The hydrocortisone conversion rates of 14 mutants were higher than that of the original strain. Among them, the hydrocortisone conversion rate of KA-91 was 42% higher than that of the original strain and reached 68.05%. The medium and fermentation conditions of KA-91 were optimized. Under the optimum condition, the hydrocortisone conversion rate reached 73.6%. Fig 7, Tab 3, Ref 5

关 键 词：酮康唑抗性 诱变育种 发酵条件 新月弯孢霉 氢化可的松 

分 类 号：TQ465[化学工程—制药化工] TQ929[轻工技术与工程—发酵工程]