c-myc反义寡核苷酸对大鼠脑胶质瘤细胞凋亡及增殖作用  

A study of effect of antisense oligonucleotides on apoptosis and proliferation in glioma in rats

作　　者：于如同[1] 白永文[1] 高文昌[1] 

机构地区：[1]徐州医学院附属医院神经外科,221002

出　　处：《江苏医药》2004年第11期801-803,i001,共4页Jiangsu Medical Journal

基　　金：江苏省卫生厅重点课题资金资助 (编号H2 0 0 10 4)

摘　　要：目的　探讨c myc反义寡核苷酸 (c mycASON)对大鼠脑胶质瘤细胞凋亡及增殖作用的影响。方法　立体定向法将C6胶质瘤细胞接种于大鼠右侧尾壳核建立鼠脑胶质瘤模型 ,采用人工合成的c mycASON和c myc正义寡核苷酸 (c mycSON)注射于瘤区。按接种后给药的种类、浓度及给药间隔时间将动物随机分成ASON 1组、2组、3组 ;SON 1组、2组、3组 ;生理盐水 1组、2组 (NS1组、2组 )。电镜观察凋亡细胞 ,流式细胞仪检测细胞凋亡率及细胞周期的变化。结果　 (1)反义组出现较多的凋亡细胞 ,正义组与生理盐水组凋亡细胞少。 (2 )各治疗组均显示凋亡峰 ,ASON 1组、2组、3组细胞凋亡率分别为 (18 8± 1 3) %、(2 0 3± 1 4 ) %、(17 8± 1 1) % ,各反义组与各正义组、各生理盐水组分别比较差异有显著性 (P <0 0 5 )。 (3)反义组肿瘤细胞在细胞周期G0 /G1期所占比例较大 ,各反义组差异无显著性 (P >0 0 5 ) ;各反义组与各正义组、各生理盐水组分别比较差异有显著性 (P <0 0 1)。结论　c myc反义寡核苷酸能诱导鼠脑胶质瘤细胞凋亡和抑制细胞增殖。Objective To explore the effect of antisense oligonucleotides on apoptosis of glioma in rats.Methods C 6 glioma cells were inoculated in right caudate nucleus in rats.The synthetic c-myc ASON and c-myc SON were injected into tumor.Then they were randomly divided into eight groups including three antisense groups,three sense groups and two saline groups according to the injected medicine,dosage and frequency.The apoptosis of the glioma was observed with eletron microscope and the apoptosis rates were analyzed with flow cytometer.Results (1)There was more apoptosis in the antisense groups than in the sense and saline groups.(2)The apoptosis cusp was found in all groups.The apoptosis rate in the ASON 1 group,ASON 2 group and ASON 3 group were (18.8±1.3)%,(20.3±1.4)% and (17.8±1.1)% respectively.There were significant differences between antisense group and sense groups and saline groups(P<0.05).(3)The majority of tumor cells in all antisense groups was retarded at G 0/G 1 cell-cycle and there was no difference in the antisense groups(P<0.05)and there were signgificant difference between antisense groups and sense groups and saline groups (P<0.01).Conclusion The c-myc ASON could induce apoptosis of glioma and suppress proliferation in rats.

关 键 词：反义寡核苷酸 凋亡细胞 脑胶质瘤细胞 生理盐水 C-MYC 大鼠 增殖作用 人工合成 细胞周期 动物 

分 类 号：R739.41[医药卫生—肿瘤] R735[医药卫生—临床医学]