宫颈上皮内瘤变染色体遗传不稳定性的研究  

Study of genetic instabilities on chromosome in cervical intraepithelial neoplasma

作　　者：刘岿然[1] 冯天达[2] 张淑兰[1] 

机构地区：[1]中国医科大学第二临床学院妇产科,辽宁沈阳110004 [2]中国医科大学第二临床学院神经外科,辽宁沈阳110004

出　　处：《中国现代医学杂志》2004年第22期77-79,共3页China Journal of Modern Medicine

摘　　要：目的分析宫颈上皮内瘤变(CIN)组织3号、10号染色体的HPV感染状态、杂合性丢失(LOH)、微卫星不稳定性(MI),以寻求CIN进展为宫颈癌的分子机制。方法选择D3S1234,D3S1300,D10S541和D10S16874个微卫星位点,应用PCR-聚丙烯酰胺-银染技术对87例CIN组织进行HPV感染状态,LOH,MI分析。结果87例CIN中,30例HPV感染阳性(34.5%);48例至少有1个位点LOH阳性(55.2%),LOH阳性率在CINⅢ明显高于CINⅠ及CINⅡ(P<0.05),在HPV感染阳性组明显高于阴性组(P<0.05);19例至少有1个位点MI阳性(37.1%),MI阳性率在CINⅢ明显高于CINⅠ及CINⅡ(P<0.05),所有MI阳性者均为LOH阳性。结论3号、10号染色体可能存在宫颈癌相关抑癌基因,LOH及MI是CIN进展为宫颈浸润癌的重要事件。Objective:To analyze infection of human papillomoviruses(HPV),loss of heterozygosity(LOH)and microsatellite instability(MI)on chromosome3and10in cervical intraepithelial neoplasma(CIN),to find the molecular mechanism of CIN to cervical invasive carcinoma.Methods:HPV infection,LOH and MI analysis of87CIN were examined with4polymorphic microsatellite markers on chromosome3and10by polymerase chain reac-tion-polyacrylamide gel-silver stain method.Results:There were30patients HPV positive out of87CIN(34.5%)LOH occurred in48(55.2%)at least one of four loci.The incidence of LOH in CINⅢis higher than that in CINⅠandⅡ,in HPV(+)is higher than in HPV(-)(P<0.05).MI occurred in19(37.1%)at least one of four loci.The in-cidence of LOH is higher in CINⅢthan in CINⅠand CINⅡ.All of the MI positive cases are LOH positive.Con-clusions:The putative suppressor genes are likely to be located on chromosome region3and10,LOH and MI may play an important role in the development from CIN to cervical invasive carcinoma.

关 键 词：宫颈上皮内瘤变 人乳头瘤病毒 杂合性丢失 微卫星不稳定性 遗传不稳定性 

分 类 号：R737.33[医药卫生—肿瘤]