端粒酶启动子肿瘤靶向基因抗大肠癌的研究  

A study on the activity of hTERT promoter related tumor targeting gene on colon cancer cell line DLD-1

作　　者：何超[1] 陈斌[1] 张磊[1] 胡文献[1] 劳伟峰[1] 黄学锋[1] 胡晓彤[1] 方炳良[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院肿瘤科,杭州310016 [2]美国MD.Anderson癌症中心

出　　处：《中华普通外科杂志》2004年第8期475-477,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目 ( 3 0 2 714 67)

摘　　要：目的探讨端粒酶 (hTERT)启动子驱动的肿瘤坏死因子相关凋亡诱导配体(TNF relatedapoptosis inducingligand ,TRAIL)基因在大肠癌细胞DLD1的表达及肿瘤杀伤作用。方法通过腺病毒载体系统将hTERT启动子驱动的TRAIL基因转入大肠癌细胞DLD1,流式细胞仪检测GFP/TRAIL的表达和DLD1细胞凋亡率。结果端粒酶启动子驱动的GFP/TRAIL基因和CMV启动子驱动的GFP(绿色荧光蛋白 )基因在DLD1内的表达率分别达 4 1 6 3%和 5 4 0 7% ;GFP/TRAIL基因对DLD1细胞的生长抑制率和凋亡率分别达 93 5 0 %和 5 6 97% ,与PBS和Ad/hTERT LacZ的差异有显著性 (P <0 0 5 )。结论端粒酶启动子驱动的GFP/TRAIL融合基因在大肠癌细胞中能够有效地表达 ;TRAIL基因对大肠癌细胞DLD1有明显的抑制生长和促凋亡作用。Objective To evaluate the expression and activity of GFP/TRAIL gene activated by the hTERT promoter on colon cancer cell line DLD-1. MethodsGFP/TRAIL gene activated by the hTERT promoter was transfected into DLD-1 with adenoviral vector,expression and apoptosis inducing ability of GFP/TRAIL protein was determined by fluorescence-activated cell sorting (FACS). [WT5”HZ]ResultsThe expression of GFP gene is 41.63% and 54.07% with either hTERT promoter or CMV promoter in DLD1 cells;GFP/TRAIL gene was able to inhibit cell growth(93.50%) and induce apoptosis(56.97%) of DLD-1 ,there was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/hTERT-LacZ)( P <0.05). ConclusionThe expression of GFP/TRAIL gene from the hTERT promoter by adenoviral vector is obvious in DLD-1 cell;GFP/TRAIL gene expression could both inhibit cell growth and induce apoptosis of colon cancer cell line DLD-1.

关 键 词：端粒酶启动子 基因治疗 大肠癌 肿瘤坏死因子 凋亡诱导配体 

分 类 号：R735.34[医药卫生—肿瘤] R730.54[医药卫生—临床医学]