Epidermal growth factor receptor pathway substrate 8 (Eps8) expression in maturing testis  

作　　者：Alain Wunsch Kai Strothmann Manuela Simoni Jorg Gromoll Eberhard Nieschlag C.Marc Luetjens 

机构地区：[1]Institute of Reproductive Medicine of the University,D-48129 Munster,Germany,Institute of Reproductive Medicine of the University,D-48129 Munster,Germany,Institute of Reproductive Medicine of the University,D-48129 Munster,Germany,Institute of Reproductive Medicine of the University,D-48129 Munster,Germany,Institute of Reproductive Medicine of the University,D-48129 Munster,Germany,Institute of Reproductive Medicine of the University,D-48129 Munster,Germany

出　　处：《Asian Journal of Andrology》2004年第3期195-203,共9页亚洲男性学杂志（英文版）

摘　　要：Aim: Although epidermal growth factor receptors are expressed in the testes, whether they signal through epidermal growth factor receptor pathway substrate 8 (Eps8) is unknown. Here we evaluated the expression pattern of Eps8 in the maturing testis. Methods: The expression of Eps8 was analysed by Northern blotting, immunocy-tochemistry and Western blotting in primary Sertoli cell cultures and in testicular tissue of rodents. Results: Eps8 is specifically expressed in gonocytes, Leydig and Sertoli cells of the neonatal rats and in Leydig and Sertoli cells of the adult rats and mice. Although gonocytes express Eps8, no signal was found in prepubertal or mature spermatogonia and the expression level of Eps8 in Sertoli cells increases with age. No regulation of Eps8 expression in primary immature rat Sertoli cells by Follicle stimulating hormone (FSH) was detected by Western blotting. Conclusion: Eps8 seems to be involved in the growth factor-controlled regulation of cell proliferation and differentiation in the seminiferous epithelium. Eps8 is a possible marker for gonocytes and in Sertoli cells it could be involved in crosstalk with other growth factor pathways.Aim: Although epidermal growth factor receptors are expressed in the testes, whether they signal through epidermal growth factor receptor pathway substrate 8 (Eps8) is unknown. Here we evaluated the expression pattern of Eps8 in the maturing testis. Methods: The expression of Eps8 was analysed by Northern blotting, immunocy-tochemistry and Western blotting in primary Sertoli cell cultures and in testicular tissue of rodents. Results: Eps8 is specifically expressed in gonocytes, Leydig and Sertoli cells of the neonatal rats and in Leydig and Sertoli cells of the adult rats and mice. Although gonocytes express Eps8, no signal was found in prepubertal or mature spermatogonia and the expression level of Eps8 in Sertoli cells increases with age. No regulation of Eps8 expression in primary immature rat Sertoli cells by Follicle stimulating hormone (FSH) was detected by Western blotting. Conclusion: Eps8 seems to be involved in the growth factor-controlled regulation of cell proliferation and differentiation in the seminiferous epithelium. Eps8 is a possible marker for gonocytes and in Sertoli cells it could be involved in crosstalk with other growth factor pathways.

关 键 词：gene expression ISOFORM Sertoli cell GONOCYTE 

分 类 号：R33[医药卫生—人体生理学]