大鼠种植型肝癌电化学治疗后细胞免疫功能的改变  

Alteration of Cellular Immune Function of Rats with Implanted Liver Tumor after Electrochemotherapy

作　　者：邵成伟[1] 王培军[1] 田建明[1] 张火俊[1] 左长京[1] 郝西彦[1] 任方远[1] 袁庆中[1] 李树平[1] 

机构地区：[1]第二军医大学长海医院放射科,上海200433

出　　处：《中国医学影像技术》2003年第5期517-518,共2页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金资助课题 (30 0 70 2 33)

摘　　要：目的　了解大鼠种植型肝癌电化学治疗 (EChT)后细胞免疫功能状态的改变。方法　应用流式细胞仪检测荷瘤大鼠治疗前后T细胞表面抗原 (CD3 、CD4、CD8)及应用免疫试剂盒检测外周血IL 2表达水平的变化 ,并与对照组进行比较。结果　荷瘤大鼠治疗前CD3 + 、CD4+ 、CD8+ 、CD4+ /CD8+ 、外周血IL 2表达水平均低于对照组。电化学治疗后CD3 + 、CD4+ 、CD8+ 、CD4+ /CD8+ 均有所回升 ,外周血IL 2水平回升。结论　荷瘤大鼠存在免疫功能低下 ,电化学治疗可纠正免疫紊乱 ,提高大鼠免疫功能。Objective To study the changes of cellular immune function in rats with implanted liver tumor after electrochemotherapy (EChT). Methods The contents of T cell phenotype in blood was detected with flow cytometry (FCM) and serum interleukin-2 (IL-2) was measured using immune kits before and after EChT.The data were compared with those in the control group. Results Before EChT,CD_3 +,CD_4 +,CD_8 +,CD_4 +/CD_8 + and IL-2 in rats liver tumor model were lower than those in the control group.After EChT,CD_3 +,CD_4 +,CD_8 +,CD_4 +/CD_8 + in the rats model increased significantly ( P <0.05),and expression of serum IL-2 increased. Conclusion The cellular immunity of rats with implanted liver tumor was suppressed,and EChT could ameliorate cellular immune function of rats.

关 键 词：大鼠 肝癌 电化学治疗 免疫功能 

分 类 号：R730.56[医药卫生—肿瘤] R735.7[医药卫生—临床医学]