灵芝菌发酵培养基的优化及灵芝胞外多糖的分离纯化  

Selection of Optimal Medium and Extraction and Purification of Ganoderma lucidum Extracellular Polysaccharide

作　　者：孙东平[1] 潘锋[1] 史小丽[1] 杨树林[1] 

机构地区：[1]南京理工大学化工学院,210094

出　　处：《中草药》2000年第12期941-943,共3页Chinese Traditional and Herbal Drugs

摘　　要：用正交法研究了不同条件对灵芝菌生长影响，从中选出了灵芝 菌液体深层发酵的优化 培养基：葡萄糖 3.6%，蛋白胨 0.4%，pH 6.0，酵母膏 0.2%，KH2PO40.1%，MgSO 4· 7H2O 0.05%，Vit B1 0.005%，每升发酵液产干菌丝体 15.6 g，粗多糖 0.72 g。 经葡 聚糖凝胶层析对灵芝胞外多糖进行了分离纯化，共有 5 个组分，并用红外光谱、紫外光谱 ，气相色谱等手段进一步分析了灵芝胞外多糖的组成，结果表明灵芝胞外多糖系由甘露糖、 果糖、葡萄糖通过糖 β-D 糖苷键构成。Optimal medium for deep layer fermentation of Ganoderma lucidum (Leyss. ex Fr. ) Karst was selected and conditions for the isolation and purification of its ex tracellular polysaccharide studied for the purpose to develop a new source for t he production of this valuable TCM. Factors influencing the growth of G. lucidum on different media were studied by orthogonal experimental de sign and polysaccharide isolated and purified by Sephadex gel filtration. The s tr uctural composition was analysed by UV, IR and GC chromatography. The best medi um for the fermentation was found to be composed of glucose 3.6%, peptone 0.4%, y east extract 0.2%, KH2PO4 0.1%， MgSO4·7H2O 0.5% and VB1 0.005%， at a pH va lue of 6.0. 15.6 g dry mycellia and 0.72 g crude polysaccharide per liter ca n be ob tained from the fermentation broth. The polysaccharide was composed of glucose, Dfructose and mannose. The method needs further scale up study.

关 键 词：灵芝菌 发酵培养基 灵芝胞外多糖 化学成分 药物研究 中药材 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]