托吡酯对戊四氮活化培养海马神经元的作用  

Effect of Topiramate on the Activation of the Cultured Hippocampal Neuron by Pentyleneterazol

作　　者：朱遂强[1] 杨美兰[1] 陈景刚 李卫华[1] 苟玉兰[1] 阮旭中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第3期350-352,F003,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No 30 170 333)

摘　　要：目的　观察戊四氮 (pentyleneterazol,PTZ)活化海马神经元前后及托吡酯干预下NF κВ活化状态的变化 ,探讨癫痫形成机制和托吡酯的作用。方法　将体外培养海马神经细胞随机分为刺激组、对照组、空白组 ,用免疫荧光化学分析方法检测NF κВ表达的变化。结果　戊四氮刺激组NF κB在核表达明显增多 ,NF κB核移位提示神经元活化 ;而托吡酯干预的对照组主要在细胞质中表达 ,提示神经元活化作用受到抑制。结论　海马神经元活化是癫痫形成机制之一 ,托吡酯可抑制神经元活化 ,这可能是其多重抗癫痫的作用机制之一。Objective To investigate the changes in NF-κB activated stage in the process of activation of hippocampal neurons by pentylenetrazol and interference of topiramate and explore the epileptogenesis and the role of topiramate.Methods The in vitro cultured hippocampal neurons were randomly divided into pentylenetrazol-activated group (experimental group), pentylenetrazol-topiramate group (control group) and blank group. By using immunofluorescence staining, the expression of NF-κB in hippocampal neurons was detected. Results In the experimental group,the expression of NF-κB in nucleus was significantly increased, indicating the neurons were activated. But in the control group, NF-κB was mainly expressed in cytoplasm, suggesting the inhibition of the neurons activation by Topiramate. Conclusion The activation of hippocampal neurons may be one of epileptogenesis. Topiramate could inhibit the activation of NF-κB in hippocampal neurons,which may be one of the antiepileptic mechanisms.

关 键 词：托吡酯 戊四氮 NF-KB 癫痫 神经元 表达 对照组 活化作用 抑制 状态 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R971[医药卫生—临床医学]