Combined Transfection with EBV-Specific Epitopes and HLA-A2 genes is More Effective than Separate Transfection in Promoting CTL Lysis against Nasopharyngeal Carcinoma  

作　　者：WeijunDing ChoylenFong 

机构地区：[1]LaboratoryofMicrobiologyandImmunology,ChengduUniversityofTraditionalChineseMedicine,37#ShierQiaoStreet,Chengdu610072,China [2]LaboratoryofImmunotherapy,DivisionofCellularandMolecularResearch,NationalCancerCentreofSingapore,llHospitalDrive169610.Singapore

出　　处：《Cellular & Molecular Immunology》2004年第3期229-234,共6页中国免疫学杂志（英文版）

摘　　要：To augment specific cytotoxic T lymphocyte(CTL)lysis is a promising strategy for cancer therapy,in this study, we examined the boosting effect of CTLs upon autologous lymphoblastoid B cell lines(LCLs)transfected with diverse plasmids,to explore the possible CTL-based immunotherapy of nasopharyngeal carcinoma(NPC). FCM analysis displayed rather high ratio(>30%)of successfully transfected LCLs by utilizing the DMRIE-C kit.CTL assays demonstrated that substantially higher ratio of CTL specific lysis was observed upon the LCLs transfected with both expression vectors encoding EBV-specific epitopes and their presentation molecule HLA-A2,in contrast with those transfected separately.By transfecting the vector encoding HLA-A2 alone,only the LCLs of HLA-A2^+ donors elicited markedly higher CTL lysis.CTL assays also showed that there existed no marked differences upon transfection by either different vectors(pcDNA3,pNGVL3 or pNGVL3-hFlex),or different EBV-derived peptides(LMP_2Pepl or LMP_2Pep2),or with or without the doubled DNA sequence encoding peptides.This study indicated a promising immunotherapy strategy on NPC through boosting and eliciting the EBV-specific CTL activation by transferring vectors encoding both EBV-specific epitopes and their presentation molecule HLA-A2 into autologous LCL,the presentation cells of MHC/peptide tetrameric complex.Cellular & Molecular Immunology.2004;1(3):229-234.To augment specific cytotoxic T lymphocyte(CTL)lysis is a promising strategy for cancer therapy,in this study, we examined the boosting effect of CTLs upon autologous lymphoblastoid B cell lines(LCLs)transfected with diverse plasmids,to explore the possible CTL-based immunotherapy of nasopharyngeal carcinoma(NPC). FCM analysis displayed rather high ratio(>30%)of successfully transfected LCLs by utilizing the DMRIE-C kit.CTL assays demonstrated that substantially higher ratio of CTL specific lysis was observed upon the LCLs transfected with both expression vectors encoding EBV-specific epitopes and their presentation molecule HLA-A2,in contrast with those transfected separately.By transfecting the vector encoding HLA-A2 alone,only the LCLs of HLA-A2^+ donors elicited markedly higher CTL lysis.CTL assays also showed that there existed no marked differences upon transfection by either different vectors(pcDNA3,pNGVL3 or pNGVL3-hFlex),or different EBV-derived peptides(LMP_2Pepl or LMP_2Pep2),or with or without the doubled DNA sequence encoding peptides.This study indicated a promising immunotherapy strategy on NPC through boosting and eliciting the EBV-specific CTL activation by transferring vectors encoding both EBV-specific epitopes and their presentation molecule HLA-A2 into autologous LCL,the presentation cells of MHC/peptide tetrameric complex.Cellular & Molecular Immunology.2004;1(3):229-234.

关 键 词：NPC LMP2 LCL HLA-A2 CTL 

分 类 号：R739.6[医药卫生—肿瘤]