前列腺癌细胞中NSBP1基因表达上调  

Up-regulated expression of a prostate cancer-related gene,NSBP1, in prostate cancer cells

作　　者：王建伟[1] 周利群[1] 杨学贞[1] 艾军魁[1] 辛殿祺[1] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院泌尿外科泌尿外科研究所,北京100034

出　　处：《基础医学与临床》2004年第4期393-397,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金 (30 3714 2 0 )

摘　　要：明确用mRNA差异显示技术 (mRNA DD)筛选出的前列腺癌相关基因NSBP1(NucleosomalBindingProtein 1)在前列腺癌细胞系的表达情况。在GenBankNR数据库中对筛选出的 5条差异表达序列标签 (EST)进行同源性分析 ,其中 1条与已知基因NSBP1高度同源 (97% )。半定量RT PCR结果显示在LNCaP、DU14 5及PC 3前列腺癌细胞系中NSBP1mRNA的表达水平分别高于正常前列腺组织 2 5倍、3 4倍和 3 6倍 ,与Northern杂交分析结果趋势一致。7例前列腺癌组织的RT PCR结果也体现了相同的表达趋势 ,NSBP1表达较配对正常前列腺组织增高 ,差异有统计学意义。以上结果表明NSBP1基因在前列腺癌细胞系和组织中的表达均明显高于正常前列腺组织 ,NSBP 1表达上调可能参与了前列腺癌的发生发展过程。To ascertain the expression level of nucleosomal binding protein gene (NSBP1) in prostate cancer cell lines. Five expressed sequence-tags chosen by mRNA differential display were used to screen the prostate cancer-related genes in the Non-redundant GeneBank of the National Center for Biotechnology Information (NCBI) by Blast program. The expression levels of NSBP1, the interested gene, was studied in the prostate cance cell lines using semiquantitative reverse transcription polymerase chain reaction(RT-PCR) and Northern Blot analysis. Four of five expressed sequence-tags expressed differentially in normal prostate and prostate cancer tissue failed to match in the Non-redundant GenBank, while the fifth one shared 97% homology with NSBP1. Compared with the normal prostate tissue, the expression levels of NSBP1 increased by 2.5-fold, 3.4-fold and 3.6-fold in LNCaP?DU145 and PC-3 cell line, respectively, which was in according with the results of Northern Blot,and the expression level of NSBP1 in 7 prostate cancer tissues was higer than that in normal ones. The paired-sample T test confirmed that the difference was significant statistically. The results suggest that the expression of NSBP1 was significantly higher in prostate cancer cell lines/tissues as compared with normal prostate tissue, which may be involved in carcinogenisis of the prostate cancer.

关 键 词：前列腺癌细胞 基因表达 正常 癌组织 上调 PT-PCR PC-3 序列标签 RNA 表达水平 

分 类 号：R737.25[医药卫生—肿瘤] R735[医药卫生—临床医学]