用昆明小鼠定量测试河豚毒素的研究  

A study on quantitative detection of tetrodotoxin with Kunming strain male mice

作　　者：张理[1] 谢克勤[2] 赵金山[1] 于丽华[2] 邢金川[1] 李培云 

机构地区：[1]山东省卫生防疫站,山东济南250014 [2]山东医科大学,山东济南250012 [3]乳山市卫生防疫站,山东乳山264500

出　　处：《中国食品卫生杂志》2004年第6期497-500,共4页Chinese Journal of Food Hygiene

摘　　要：为建立昆明小鼠定量测定河豚毒素(TTX)方法,选用雄性昆明小鼠进行研究,结果显示TTX致昆明小鼠的LD50为0165μg,TTX剂量在045～256μg之间与昆明小鼠平均死亡时间成线性关系;当河鱼样品提取液TTX浓度低于045μgml时,腹腔注射1ml提取液致小鼠平均死亡时间大于4min,在此范围内与ICR小鼠进行比较,毒素检测结果基本一致。此外对该传统实验方法进行了无需小鼠体重校正、利用平均数计算等项改进。用昆明小鼠定量测试河豚毒素既方便又可靠。To establish laboratory methodology for the quantitative detection of tetrodotoxin (TTX), a bioassay was carried out using Kunming strain male mice. The results indicated that the 50% lethal dose (LD 50 ) of TTX for Kunming mice was 0 165 μg. A linear relationship between the TTX dose (0 45～2 56 μg) and the mean time of death in Kunming mice was observed When TTX concentration in globefish extracts was lower than 0 45 μg/ml, the mean time of death of mice was 4～30 minutes. This coincided with the results of TTX detection using ddy mice or ICR mice. The results of the study suggest that the quantitative detection of TTX with Kunming mice is convenient and reliable.

关 键 词：河豚毒素 昆明小鼠 TTX 定量 ICR小鼠 腹腔注射 检测结果 样品提取 LD50 浓度 

分 类 号：R114[医药卫生—卫生毒理学]